林业科学研究2025，Vol.38Issue(5)：1-13,13.DOI:10.12403/j.1001-1498.20240510
过量表达MYB158基因显著抑制'741'杨插条的不定根形成
Overexpression of MYB158 Gene in'741'Poplar Significantly Inhibited Adventitious Root Formation of Cuttings
摘要
Abstract
[Objective]Poplar is widely used in timber forest and ecological protection forest construction,and has important economic and ecological value.Poplar superior clones are mainly propagated through cuttings,so uncovering the regulatory mechanism underlying adventitious root formation is of great signific-ance for improving the reproductive efficiency of poplar trees.[Method]In our previous studies,we identi-fied MYB158,a secondary cell wall-related transcription factor from'741'poplar.In this study,we tested the rooting ability of cuttings from MYB158 overexpressing'741'poplars.To investigate the molecular mechanisms by which MYB158 regulates adventitious root formation,we performed auxin content determ-ination,exogenous IAA treatment,and conducted gene expression analysis.[Result]This study found that overexpression of MYB158 significantly impaired rooting ability under normal condition.By the 7th day after cutting,the rooting rate of transgenic trees decreased by 53%to 73%compared to the wild type.The auxin content in the stems of MYB158 transgenic poplar was significantly reduced.Exogenous application of IAA was able to alleviate the inhibition of MYB158 on adventitious root formation,and the rooting rate was increased by more than 70%compared to untreated cuttings.Gene expression analysis revealed that MYB158 inhibited the expression of auxin transporters.[Conclusion]Overexpression of MYB158 hinders the polar transport of auxin in the stem by repressing the expression of auxin transporters,thereby inhibit-ing adventitious root formation.These findings provide new insights into the regulatory mechanisms of poplar adventitious root formation.关键词
'741'杨/MYB158/不定根形成/生长素/生长素转运蛋白Key words
'741'poplar/MYB158/adventitious root formation/auxin/auxin transporters分类
农业科技引用本文复制引用
姜鹏飞,韩欢,曾庆银,刘妍婧..过量表达MYB158基因显著抑制'741'杨插条的不定根形成[J].林业科学研究,2025,38(5):1-13,13.基金项目
国家自然基金青年科学基金项目(32101552) （32101552）
"十四五"国家重点研发计划课题(2022YFD2200102) （2022YFD2200102）
