绿洲农业科学与工程2025，Vol.10Issue(4)：44-49,6.DOI:10.26941/j.cnki.2096-2177.2025.04.007
"华金"油茶启动培养体系初探
Preliminary Research on the Initiation Culture System of"Hua jin"Camellia oleifera
摘要
Abstract
In order to establish an in vitro regeneration system for'Hua jin'Camellia oleifera,this study compared the parameters of different explants(leaves,stem segments,and floral organs),sterilizing time(6-13 min),and hormones(6-BA,PIC),and thus We then screened out the suiTab.combinations of explants,sterilization time and hormones for in vitro regeneration of'Hua jin'Camellia oleifera.The results showed that leaves and flower organs were the best explants for'Huajin'Camellia oleifera,and the optimal sterilization time was 8 min and 7 min,respectively,and the stem segments were not easy to regenerate.5.0 mg·L-1 6-BA+2.0 mg L-1 PIC was the optimal hormone combination for leaf regeneration,and 3.0 mg·L-1 6-BA+2.0 mg·L-1 PIC was was the best hormone combination for the regeneration of floral organs,and the proliferation of floral organ healing tissues was better after subculture.In conclusion,it can be seen that the floral organs and leaves are the best explants for the starter culture of'Hua jin'Camellia oleifera,and the combination of 6-BA and PIC is good for the induction of its healing tissues,and the healing tissues originated from the floral organ explants proliferated better and dedifferentiated thoroughly.关键词
"华金"油茶/离体再生/启动培养Key words
"Hua jin"Camellia oleifera/in vitro regeneration/initial culture分类
农业科技引用本文复制引用
倪天虹,张贺,张旭阳,岳建华,张琰.."华金"油茶启动培养体系初探[J].绿洲农业科学与工程,2025,10(4):44-49,6.基金项目
信阳农林学院青年教师科研基金项目(QN2022019) （QN2022019）
河南省科技攻关项目(252102110233) Funded by the project of Scientific Research Fund for Young Teachers of Xinyang Agriculture and Forestry University(QN2022019) （252102110233）
The project of Science and Technology Research Project of Henan Province(252102110233) （252102110233）
