煤田地质与勘探2025，Vol.53Issue(10)：23-32,10.DOI:10.12363/issn.1001-1986.25.06.0417
排土场土层重构与接菌对植物水分利用的影响机制
Influence mechanisms of soil layer reconstruction and fungal inoculation on plant water use in waste dumps
摘要
Abstract
[Objective]In the mining areas of open-pit coal mines in the arid and semi-arid regions in western China,wa-ter scarcity severely restricts ecological restoration.As preliminary ecological effects have been achieved through soil layer reconstruction and bioremediation-based reclamation technologies in waste dumps of the mining areas,there is an urgent need to investigate the effects of both technologies on the water use and growth of representative plants.This will provide a scientific basis for accelerating ecological restoration in the mining areas.[Methods]In a waste dump of the Heidaigou open-pit coal mine in Inner Mongolia,four experimental program for soil layer treatment were conducted,i.e.,three-layer structured soils with no fungal inoculation(C-CK),three-layer structured soils with Arbuscular mycor-rhizal fungi(AMF)inoculation(C-AM),mixed soils with AMF inoculation(D-AM),mixed soils with no fungal inocula-tion(D-CK),with Amorpha fruticosa used as the test plant.Using in situ field experiments on soil water evaporation and infiltration,combined with hydrogen and oxygen isotope analysis of water,this study investigated water distribution in reconstructed soil layers combined with bioremediation-based reclamation and explored the effects of the combination on plant water use.Besides,the primary factors influencing the water use efficiency(WUE)and plant height were identi-fied using variance analysis and principal component analysis.[Results and Conclusions]In the waste dump,three-lay-er structured soils effectively reduced soil water evaporation,and the underlying impermeable layer prevented water leakage,thereby achieving water retention.Specifically,the overall water storage of the three-layer structured soils in the C-CK and C-AM zones proved 9.1％and 8.37％higher than that of the mixed soils,respectively.After reclamation,AMF inoculation accelerated water infiltration,reduced evaporation,and increased the water consumption of Amorpha fruticosa.Furthermore,AMF inoculation enhanced the WUE of Amorpha fruticosa by promoting root development and photosynthesis,with the WUE of the C-AM treatment measuring 1.18,1.40,and 2.30 times that of the C-CK,D-AM,and D-CK treatments,respectively.The growth indicators of Amorpha fruticosa under the four treatments decreased in the order of C-AM,D-AM,C-CK,and D-CK.The three-layer structure of soils enables the effective retention of soil moisture.This structure,combined with AMF inoculation,can promote the root development and photosynthetic rate of Amorpha fruticosa,thus enhancing its WUE under limited water resources.The reclamation technology integrating three-layer structured soil and AMF inoculation can serve as an ecological reclamation measure for the waste dumps of open-pit coal mines in western China,thereby contributing to the sustainable development of green mines.关键词
丛枝菌根真菌(AMF)/土层重构/氢氧同位素/水分利用效率/排土场/紫穗槐/黑岱沟露天煤矿Key words
Arbuscular mycorrhizal fungi(AMF)/soil layer reconstruction/hydrogen and oxygen isotopes/water use ef-ficiency(WUE)/waste dump/Amorpha fruticosa/Heidaigou open-pit coal mine分类
矿山工程引用本文复制引用
毕银丽,李中庆,柯增鸣,彭苏萍..排土场土层重构与接菌对植物水分利用的影响机制[J].煤田地质与勘探,2025,53(10):23-32,10.基金项目
国家自然科学基金重大项目(52394195) （52394195）
