农业生物技术学报2025，Vol.33Issue(11)：2348-2363,16.DOI:10.3969/j.issn.1674-7968.2025.11.002
小麦ADP-葡萄糖转运蛋白BT1家族基因表达与优异等位基因分析
Gene Expression and Elite Alleles Analysis of the ADP-glucose Transporter BT1 Family in Wheat(Triticum aestivum)
摘要
Abstract
BRITTLE 1(BT1)mediates the unidirectional transmembrane transport of ADP-glucose(ADP-Glc),which is critical in starch synthesis.However,systematic studies on the members of the BT1 gene family and their biological functions remain lacking in wheat(Triticum aestivum).In this study,genome-wide identification,gene expression pattern,and elite alleles analysis of the BT1 family genes were conducted in wheat.Phylogenetic analysis,protein structure characterization,prediction of cis-acting elementin promoters,and qRT-PCR were employed to investigate the evolutionary relationships and expression patterns of BT1 family genes in wheat.Additionally,elite alleles of TaBT1 family members were screened.Phylogenetic and structural analysis indicated that the wheat TaBT1 protein shared a close evolutionary relationship with barley(Hordeum vulgare)HvBT1 protein.A total of 9 TaBT1 genes were identified in the wheat genome,all of encoding proteins containing the Mito-carr domain.Moreover,members within the same group exhibited similar conserved protein domains,indicating high evolutionary conservation of the TaBT1 gene family.The promoter regions of the TaBT1 genes contained various plant growth and development related cis-acting elements.TaBT1 genes were predominantly highly expressed in wheat grains,with the highest expression levels detected in the endosperm.Among the TaBT1 gene family members,8 TaBT1 genes exhibited distinct alleles,with 3 showing alleles significantly associated with grain traits.Elite alleles of TaBT1-1A and TaBT1-1B were showed clear geographical differentiation across major wheat producing regions.This study provides a theoretical foundation for the biological function of the TaBT1 genes in regulating wheat grain development and their potential application in elite allele-based breeding.关键词
小麦/BRITTLE1(BT1)/生物信息学/表达分析/等位基因Key words
Wheat/BRITTLE1(BT1)/Bioinformatics/Expression analysis/Allele分类
农业科学引用本文复制引用
员乐彤,陈涛,郭利建,车卓,田甜,张艳艳,王鹏,杨德龙..小麦ADP-葡萄糖转运蛋白BT1家族基因表达与优异等位基因分析[J].农业生物技术学报,2025,33(11):2348-2363,16.基金项目
甘肃省农业农村厅种业攻关项目(GYGG-2024-2) （GYGG-2024-2）
甘肃省科技厅基础研究创新群体项目(24JRRA633) （24JRRA633）
中央引导地方科技发展资金(23ZYQA0322) （23ZYQA0322）
甘肃省高校科研创新平台重点培育项目(2024CXPT-01) （2024CXPT-01）
甘肃省科技重大专项(22ZD6NA009) （22ZD6NA009）
国家自然科学基金(32260520 （）
32360518 （）
32401920) （）
甘肃省高等学校产业支撑计划(2022CYZC-44) （2022CYZC-44）
甘肃省教育厅青年博士支持项目(2024QB-062) （2024QB-062）
省部共建干旱生境作物学国家重点实验室开放基金(GSCS-2023-07) （GSCS-2023-07）
