农业生物技术学报2025，Vol.33Issue(11)：2364-2377,14.DOI:10.3969/j.issn.1674-7968.2025.11.003
OsPRK通过代谢重编程增强水稻耐旱与稻瘟病抗性
OsPRK Enhances Drought Tolerance and Blast Resistance in Rice(Oryza sativa)via Metabolic Reprogramming
摘要
Abstract
Phosphoribulokinase(PRK)is a key enzyme in the Calvin cycle of photosynthetic reactions and plays a central role in carbon assimilation.Recent studies suggest that PRK may also participate in plant responses to abiotic and biotic stresses,but its molecular mechanisms under stress conditions remain unclear.This study investigated the regulatory mechanism of OsPRK in response to drought and rice blast fungus(Magnaporthe oryzae)stress by generating O.sPRK-overexpressing transgenic rice lines and combining metabolomic analysis with phenotypic characterization.Using Agrobacterium-mediated genetic transformation and qPCR,we successfully obtained T2 generation homozygous transgenic rice plants(Oryza sativa ssp.japonica cv.Nipponbare)carrying a single-copy transgene and exhibiting significantly higher OsPRK expression than wild-type(WT)plants.High-performance liquid chromatography-tandem mass spectrometry approach were employed to analyze metabolites in three-leaf-stage seedlings from transgenic and WT plants,identifying a total of 2 036 metabolites.Orthogonal partial least squares-discriminant analysis screened 136 significantly differentially accumulated metabolites(DAMs),including 93 that were upregulated and 43 that were downregulated.These DAMs were primarily enriched in metabolic pathways potentially related to stress response,such as isoflavonoid biosynthesis,flavonoid biosynthesis,and phenylpropanoid biosynthesis.Phenotypic analysis under stress conditions showed that OsPRK-overexpressing plants exhibited delayed leaf wilting under drought stress and had approximately 17％higher survival rate than WT plants after rehydration.After inoculation with Magnaporthe oryzae,transgenic plants developed a significantly fewer lesions,indicating enhanced resistance.Furthermore,key photosynthetic parameters at the mature stage,including net photosynthetic rate and stomatal conductance,were not significantly affected,demonstrating that OsPRK overexpression enhances stress tolerance without compromising photosynthetic efficiency.Interestingly,it was observed that T0 generation osprk mutant plants exhibited significantly lower chlorophyll content during the seedling hardening stage compared with WT and overexpressing lines,and eventually died.This study reveals a novel mechanism by which OsPRK regulates stress resistance in rice through metabolic networks remodeling,providing a theoretical foundation for molecular breeding of stress-tolerant crops.关键词
水稻/水稻磷酸核酮糖激酶(OsPRK)/代谢组学/干旱胁迫/稻瘟病Key words
Oryza sativa/Oryza sativa phosphoribulokinase(OsPRK)/Metabolomics/Drought stress/Rice blast分类
农业科技引用本文复制引用
何炜,周平,魏毅东,崔丽丽,蔡秋华,谢鸿光,谢华安,张建福..OsPRK通过代谢重编程增强水稻耐旱与稻瘟病抗性[J].农业生物技术学报,2025,33(11):2364-2377,14.基金项目
福建省自然科学基金(2023J01356) （2023J01356）
中央引导地方科技发展专项(2022L3018) （2022L3018）
福建省人民政府-中国农业科学院协同创新工程"5511"(XTCXGC2021019) （XTCXGC2021019）
福建省财政专项-福建省农业科学院科技创新团队(CXTD2021005-3) （CXTD2021005-3）
福建省科技重大专项(2024NZ029027) （2024NZ029027）
