农业生物技术学报2025，Vol.33Issue(11)：2428-2440,13.DOI:10.3969/j.issn.1674-7968.2025.11.008
小黄鱼eif2ak3和chop基因的克隆及其对高温胁迫的响应特征
Cloning of eif2ak3 and chop and Their Response Characteristics to Heat Stress in Small Yellow Croaker(Larimichthys polyactis)
摘要
Abstract
Fish are typical ectothermic animals.Under high temperatures,fish are susceptible to endoplasmic reticulum(ER)stress,which activates the eukaryotic translation initiation factor 2α kinase 3(EIF2AK3)/C/EBP-homologous protein(CHOP)signaling pathway,thereby inducing apoptosis and tissue damage.To investigate the response characteristics of eif2ak3 and chop in small yellow croaker(Larimichthys polyactis)under high-temperature stress,this study used small yellow croaker as the research subject and cloned the CDS of eif2ak3 and chop.The CDS of eif2ak3 was cloned,comprising 3 321 bp and encoding 1 106 amino acids,with conserved domains STKc_EIF2AK3_PERK and Luminal_EIF2AK3.Additionally,the CDS of chop was obtained,spanning 939 bp and encoding 263 amino acids,featuring a single BRLZ conserved domain.Phylogenetic analysis indicated that eif2ak3 and chop in the small yellow croaker exhibit the closest evolutionary relationship with their orthologs in the large yellow croaker(L.crocea).qPCR analysis revealed that eif2ak3 and chop were ubiquitously expressed across various tissues of the small yellow croaker,albeit with significant tissue-specific variation.Specifically,eif2ak3 expression was highest in the liver,whereas chop expression was highest in the heart.Under heat stress at(32±0.5)℃,the liver expression profiles of eif2ak3 and chop were synchronized,peaking immediately upon reaching 32 ℃ and declining progressively thereafter.This suggested that eif2ak3 and chop playing a critical regulatory role during the initial phase of the heat stress response.This study reveals the expression characteristics of eif2ak3 and chop in response to heat stress,providing important data for further elucidating the molecular mechanisms of fish response to heat stress.关键词
小黄鱼/真核翻译始动因子2α激酶3(EIF2AK3)/C/EBP同源蛋白(CHOP)/高温胁迫/基因表达Key words
Larimichthys polyactis/Eukaryotic translation initiation factor 2α kinase 3(EIF2AK3)/C/EBP-homologous protein(CHOP)/Heat stress/Gene expression分类
农业科技引用本文复制引用
刘四芳,刘峰,张天乐,李倩,刘浩文,朱家杰,杨倩倩,楼宝..小黄鱼eif2ak3和chop基因的克隆及其对高温胁迫的响应特征[J].农业生物技术学报,2025,33(11):2428-2440,13.基金项目
浙江省"三农九方"科技计划(2025SNJF096) （2025SNJF096）
国家自然科学基金(32102765) （32102765）
浙江省重点研发计划(2021C02055) （2021C02055）
