农业生物技术学报2025，Vol.33Issue(11)：2460-2471,12.DOI:10.3969/j.issn.1674-7968.2025.11.010
贝莱斯芽胞杆菌Y-2-3对芭蕉炭疽菌的拮抗作用及其对香蕉炭疽病的防效
The Antagonistic Effect of Bacillus velezensis Y-2-3 on Colletotrichum musae and Its Efficacy in Preventing Banana Anthracnose Disease
摘要
Abstract
The banana(Musa acuminata)anthracnose disease caused by Colletotrichum musae is one of the most common post harvest diseases of bananas.To screen biocontrol bacterial strains with significant efficacy against banana anthracnose,in this study,a strain,Y-2-3,was isolated from soil samples collected from an abandoned salt field in Beihai,Guangxi,China,showing an inhibition rate of 75.62％against C.musae,which mycelia treated with Y-2-3 became swollen,deformed,and intertwined.Functional characterization revealed that Y-2-3 possesses growth-promoting properties,including nitrogen fixation,ammonia(NH3)production,and phosphate solubilization,as well as the ability to produce cellulase and amylase.16S rRNA and gyrase subunit B(gyrB)gene sequencing identified Y-2-3 as Bacillus velezensis.Dual culture assays confirmed that Y-2-3 exhibited broad-spectrum antifungal activity,with inhibition rates exceeding 50％against multiple pathogenic fungi.Moreover,volatile compounds produced by Y-2-3 exhibited a strong inhibitory effect on C.musae.Postharvest preservation experiments demonstrated that banana disease lesions were controlled within 40％under volatile treatment with Y-2-3.In conclusion,strain Y-2-3 exhibits both growth-promoting and biocontrol properties,showing great potential for application in banana anthracnose management and sustainable agriculture.This study provides theoretical basis and high-quality bacterial resources for the biological control of banana anthracnose.关键词
贝莱斯芽胞杆菌/香蕉炭疽病/芭蕉炭疽菌/生物防治Key words
Bacillus velezensis/Banana anthracnose/Colletotrichum musae/Biological control分类
农业科技引用本文复制引用
谢晓莹,廖克妙,花晓婕,陆玉琴,蒙丽林,尹闻力,黄文善,姜明国,周燕..贝莱斯芽胞杆菌Y-2-3对芭蕉炭疽菌的拮抗作用及其对香蕉炭疽病的防效[J].农业生物技术学报,2025,33(11):2460-2471,12.基金项目
广西民族大学2020年度校级引进人才科研启动项目(2020KJQD23) （2020KJQD23）
