中国海洋大学学报（自然科学版）2025，Vol.55Issue(11)：42-51,10.DOI:10.16441/j.cnki.hdxb.20240314
不同溶解氧水平对鳗草种子萌发和实生苗建成的影响
Effects of Different Levels of Dissolved Oxygen on Seed Germination and Seedling Establishment of Zostera marina
摘要
Abstract
To clarify the optimal dissolved oxygen(DO)level for promoting eelgrass(Zostera marina)seed germination and seedling establishment,the cumulate germination rate,germination index,and nutrient content of eelgrass of seed germination stage,as well as the survival rate,growth rate,morphological indexes and leaf photosynthetic pigment content of the seedlings were assessed at five DO levels(2,4,6,8(control),and 10 mg·L-1).The physiological responses of eelgrass seed germination and seedling establishment under different dissolved oxygen levels were analyzed.The experiment was carried out for 110 d,60 d for seed germination and 50 d for seedling cultivation.The results were as follows:(1)DO level had a significant effect on the cumulate germination rate and germination index of eelgrass seeds(P＜0.05),and the cumulate germination rate,germination index,and vitality index of seeds in the 2 mg·L-1 treatment group were 1.1 to 1.5 times greater than those of the control group at 60 d;(2)α-amylase activity in all treatment groups significantly increased(P＜0.05)during the early stage of seed germination(0～20 d)and the seed moisture and soluble sugar content significantly increased in the rapid germination stage(20～40 d)while the total protein content and starch contents significantly decreased(P＜0.05).The water and soluble sugar content in the 2 mg·L-1 treatment group were 1.1 and 1.4 times higher than those of the control group,and the total protein content and starch contents were 85.5％and 90.7％of those of the control group,respectively;(3)different levels of DO during the seed germination stage also significantly affected the survival rate,growth rate,and chlorophyll content of the leaves(P＜0.05).After 50 days of cultivation,the survival rate,growth rate,and chlorophyll a/b ratio of seedlings in the 2 mg·L-1 treatment group reached their highest values,which were 1.2 to 1.9 times higher than those of the control group;(4)seed starch content exhibited a significant negative correlation with seed germination and seedling survival.In contrast,seed water and total protein content were significantly and positively correlated with seed germination and seedling survival(P＜0.05).These findings indicated that the optimal DO level to promote eelgrass seed germination and seedling growth was 2 mg·L-1.This level of DO enhanced α-amylase activity in eelgrass seeds and accelerated starch decomposition,which in turn increased water absorption and expansion of the seeds as well as accumulation of soluble sugars,and thus promoted seed germination as well as the survival,growth,and photosynthetic capacity of seedlings.These results provided a scientific basis for enriching the seagrass propagation biology and enhancing the effectiveness of seagrass seedling-based ecological restoration.关键词
溶解氧/鳗草/累积萌发率/实生苗存活率/营养物质Key words
dissolved oxygen/Zostera marina/cumulate germination rate/seedling survival rate/nu-trient substance分类
农业科技引用本文复制引用
杨启文,龙庆刚,遆丽军,袁铂韬,田璐,张沛东..不同溶解氧水平对鳗草种子萌发和实生苗建成的影响[J].中国海洋大学学报（自然科学版）,2025,55(11):42-51,10.基金项目
国家自然科学基金项目(42076100) （42076100）
中国海洋大学-中国船舶集团环境发展有限公司海洋生态保护修复与可持续利用技术联合研究中心项目(H20240008) （H20240008）
威海市天鹅湖-养鱼池湾海草床生态修复及科研实验项目(H20240055)资助Supported by the National Natural Science Foundation of China(42076100) （H20240055）
the Project of Joint Research Center for Conservation,Restoration & Sustainable Utilization of Marine Ecology of Ocean University of China-China State Shipbuilding Corporation Environ-mental Development Company Limited(H20240008) （H20240008）
the Seagrass Bed Ecological Restoration and Scientific Research Experiment Project in Swan Lake-Yangyuchi Bay of Weihai(H20240055) （H20240055）
