中国海洋大学学报（自然科学版）2025，Vol.55Issue(11)：62-72,11.DOI:10.16441/j.cnki.hdxb.20240238
基于响应面法的网状鼻胞藻S6A去除海水中正丙苯和异丙苯的环境条件优化
Optimization of the Environmental Condition for the Removal of n-Propylbenzene and Isopropylbenzene in Seawater by Rhinomonas reticulata S6A Based on Response Surface Methodology
摘要
Abstract
N-propylbenzene(n-PBZ)and isopropylbenzene(i-PBZ)are considered hazardous and noxious substances,and once leaked into the sea,they will cause serious harm to the marine ecosystems.Our previous study has found that the removal rate of n-PBZ and i-PBZ at a concentration of 5 mg/L by Rhodomonas reticulata S6A within 7 days were 34.6％and 38.8％,respectively.To further clarify the suitable environmental conditions for the remediation of propylbenzene-contaminated seawaters,the effects of salinity,temperature and light intensity on the removal of n-PBZ and i-PBZ from seawater by R.reticulata S6A were investigated in this study using response surface methodology(RSM)based on the Box-Behnken design.The results indicated that light intensity and temperature were the key factors affecting the removal of n-PBZ and i-PBZ,respectively.Additionally,the interactions between temperature and salinity,as well as temperature and light intensity,were found to be significant.The optimal environmental condition for the microalga to remove n-PBZ was as follows,salinity 27.2,temperature 25.1℃ and light intensity 237.9 μmol/(m2·s)with a measured removal rate of 51.2％.The most suitable environmental condition for removing i-PBZ was as follows,salinity 28.4,temperature 25.5℃ and light intensity 580.8 μmol/(m2·s)with a measured removal rate of 54.6％.The removal rate prediction model constructed based on the experimental results could be used to estimate the bioremediation effect in propylbenzene-contaminated seawaters,providing a decision-making basis for determining the appropriate occasions and times for using the microalga.关键词
网状鼻胞藻/正丙苯/异丙苯/响应面法/环境因子/海水Key words
Rhinomonas reticulata/n-propylbenzene/isopropylbenzene/response surface methodolo-gy/environmental factors/seawater分类
资源环境引用本文复制引用
崔嘉丽,李玉梅,冷雨,孟范平..基于响应面法的网状鼻胞藻S6A去除海水中正丙苯和异丙苯的环境条件优化[J].中国海洋大学学报（自然科学版）,2025,55(11):62-72,11.基金项目
国家自然科学基金项目(42077335)资助Supported by the National Natural Science Foundation of China(42077335) （42077335）
