中国海洋大学学报（自然科学版）2025，Vol.55Issue(11)：73-88,16.DOI:10.16441/j.cnki.hdxb.20240116
澳洲异尾涡虫再生过程的基因共表达网络及关键基因的调控模式分析
Analysis of Gene Co-Expression Networks and Regulatory Patterns of Key Genes During Regeneration in Heterochaerus australis
摘要
Abstract
As a typical animal of Acoela,Heterochaerus australis possesses remarkable regenerative a-bilities.Thus,the research of the regeneration process is helpful to enrich the understanding of the mo-lecular mechanism of the regeneration process of primitive bilateria.In this research,we used weighted gene co-expression network analysis(WGCNA)on transcriptomic data from regeneration process of head and tail tissue in H.australis to analyze the key gene regulatory networks during head and tail tissue regeneration.Through differential gene expression analysis and similarity analysis of WGCNA modules,we identified similar WGCNA modules between head and tail tissues regeneration.KEGG enrichment a-nalysis revealed that the function of genes in these key modules are associated with cell cycle,DNA rep-lication,the signaling pathways related to the maintenance of cell energy balance and the inhibition of abnormal cell proliferation,indicating these modules are involved in maintaining the correct regeneration of head and tail tissues of H.australis.Based on the gene annotation and expression patterns of picked hub genes in these key modules,this research further analyzed the cascading expression patterns of the key gene regulatory networks during head and tail tissue regeneration in H.australis.关键词
澳洲异尾涡虫/加权基因共表达网络分析(WGCNA)/转录组/再生/基因共表达模块/枢纽基因/差异表达基因Key words
Heterochaerus australis/weighted gene co-expression network analysis(WGCNA)/tran-scriptome analysis/regeneration/WGCNA module/hub gene/differentially expressed gene分类
生物科学引用本文复制引用
蒲忠棋,姚立杰,常欣瑶,孙雪,李语丽..澳洲异尾涡虫再生过程的基因共表达网络及关键基因的调控模式分析[J].中国海洋大学学报（自然科学版）,2025,55(11):73-88,16.基金项目
国家自然科学基金项目(32222085)资助Supported by the National Natural Science Foundation of China(32222085) （32222085）
