蔬菜Issue(11)：36-41,6.DOI:10.26924/j.cnki.sczz.2025.1104
基于RNA-seq的番茄果实维生素C含量代谢通路分析
Metabolic Pathway Analysis of Vitamin C Content in Tomato Fruits Based on RNA-seq
摘要
Abstract
Vitamin C(AsA)in tomato fruits,as a core antioxidant,significantly impacts fruit quality and postharvest storage characteristics.Using tomato germplasms T248054 and T248037,which exhibit significant differences in AsA content,transcriptome sequencing and bioinformatics analysis were conducted to elucidate the molecular mechanisms and metabolic regulation underlying AsA biosynthesis in ripe tomato fruits.Transcriptome sequencing identified 3 156 differentially expressed genes(DEGs).KEGG enrichment analysis revealed that these DEGs were significantly enriched in seven pathways,including Photosynthesis(sly00 195),Glutathione metabolism(sly00480)and Phenylpropanoid biosynthesis(sly00940).Further analysis focusing on the Glutathione metabolism pathway(sly00480)showed that the expression of L-ascorbate peroxidase 3(APX3,101264282)was significantly downregulated(log2FC=-2.12)in T248054,potentially reducing the oxidative consumption of AsA.Concurrently,dehydroascorbate reductase(DHAR1,101257804)was significantly upregulated(log2FC=1.97),enhancing the regeneration of AsA from dehydroascorbate.The widespread downregulation of Glutathione S-transferase(GSTs)genes might indirectly promote AsA accumulation by stabilizing the glutathione(GSH)pool.The bidirectional regulation of the AsA-GSH cycle-reducing consumption and enhancing regeneration in T248054 is likely the key mechanism for its significantly increased vitamin C content.This study provides a theoretical basis for targeted optimization of the antioxidant metabolic network in tomatoes and for breeding varieties of high content of vitamin C.关键词
番茄/维生素C/转录组/AsA-GSH循环/代谢通路/生物信息学Key words
tomato/Vitamin C/transcriptome/AsA-GSH cycle/metabolic pathway/bioinformatics引用本文复制引用
郁伟峰,高明刚,李金玲,张翠兰,梁溪原,杨朝霞,袁艳芳,曹慧,梁增文..基于RNA-seq的番茄果实维生素C含量代谢通路分析[J].蔬菜,2025,(11):36-41,6.基金项目
山东省科技型中小企业创新能力提升工程项目(2023TSGC0762) （2023TSGC0762）
2024年大学生创新创业训练计划项目(S202411067014). （S202411067014）
