水产学报2025，Vol.49Issue(12)：21-30,10.DOI:10.11964/jfc.20240514507
斑马鱼seipin基因克隆及其在肝脏脂质代谢中的作用
Cloning of zebrafish(Danio rerio)seipin gene and its role in D.rerio liver lipid metabolism
摘要
Abstract
Seipin is a key protein regulating lipid storage.Mutation in the bscl2 gene encoding seipin causes severe congenital generalized lipoatrophy in humans.Patients lose whole-body adipose tissue,accompanied by abnormal liver fat deposition.The function of seipin in adipose tissue formation and adipocyte differentiation has been widely studied.However,how seipin regu-lates lipid storage and metabolism in the liver is still unclear.Therefore,in order to explore the role of fish lipid droplet-related protein seipin in liver lipid metabolism,the coding sequence of seipin was cloned for sequence analysis,and the expression of triglyceride(TG)metabolism-related genes in Danio rerio liver and liver cells was detected through real-time fluorescence quantitative PCR(RT-qPCR)after interfering and overexpressing seipin in vivo and in vitro experiments.The results showed that the seipin coding sequence of D.rerio is 1 053 bp in length,encoding 350 amino acids.Bioinformatics analysis showed that D.rerio seipin protein belongs to alkaline,unstable and hydrophobic transmembrane protein.RT-qPCR results showed that compared with the control group,the expression of TG synthesis-related gene dgat1b in D.rerio liver and liver cells was signi-ficantly increased(P<0.05),the expression of TG transport-related gene mttp was significantly increased(P<0.05),the expres-sion of TG lipolysis related gene lpl was increased(P>0.05),and the expression of atgl was decreased(P>0.05).After 36 h of seipin interference,the expression of TG synthesis-related gene dgat1a in D.rerio liver and liver cells increased(P>0.05),the expression of mttp decreased significantly(P<0.05),and the expression of lpl decreased significantly(P<0.05).After overex-pression of seipin for 24 h,the expression of TG synthesis-related gene dgat2in D.rerio liver cells decreased significantly(P<0.05),the expression of mttp decreased significantly(P<0.05),and there was no significant difference in the expression of lpl(P>0.05).After overexpression of seipin for 36 h,the expression of TG synthesis-related gene dgat2 in D.rerio liver cells increased significantly(P<0.05),the expression of mttp increased significantly(P<0.05),and there was no significant differ-ence in the expression of lpl(P>0.05).The above results indicate that seipin may participate in D.rerio liver lipid metabolism by affecting the expression of TG metabolism-related genes.This study provides a basis for further study on the mechanism of seipin regulating lipid storage and metabolism in D.rerio.关键词
斑马鱼/seipin/肝脏/脂质代谢Key words
Danio rerio/seipin/liver/lipid metabolism分类
生物学引用本文复制引用
李伟嘉,沈亚男,杜健龙,麦康森,艾庆辉..斑马鱼seipin基因克隆及其在肝脏脂质代谢中的作用[J].水产学报,2025,49(12):21-30,10.基金项目
国家自然科学基金(31830103) National Natural Science Foundation of China(31830103) （31830103）
