水产学报2025，Vol.49Issue(12)：31-39,9.DOI:10.11964/jfc.20240414464
setd7对尼罗罗非鱼的低氧耐受能力的影响
Effect of setd7 on hypoxia tolerance in Nile tilapia(Oreochromis niloticus)
摘要
Abstract
This study aims to explore the function of the methyltransferase gene setd7 in hypoxia tolerance in fish,using Nile tilapia(Oreochromis niloticus)as the experimental model.By employing gene editing technology,we constructed setd7 gene mutation lines to assess their physiological responses and tolerance capabilities in low oxygen environments,providing theoret-ical basis and experimental materials for molecular breeding of hypoxia tolerant fish and research on the functional mechan-isms of methyltransferases.Using CRISPR/Cas9 gene editing technology,specific knockout targets were designed for the con-served amino acid region of the second exon of the setd7 gene in O.niloticus,and microinjection was performed on one cell fertilized eggs.The gene editing efficiency was verified through T7E1 enzyme digestion experiments and sequencing analysis,successfully obtaining the setd7+/-mutation line with a-23 bp base deletion.Further,one month old F1 generation tilapia were subjected to hypoxic stress treatment to observe their hypoxia characteristics and survival time,assessing their hypoxia toler-ance.The experimental results showed that the setd7+/-mutation line exhibited a loss of function of the SETD7 protein due to a frameshift mutation,with a significantly reduced expression level of the setd7 gene compared to the wild type.In the hypoxic stress experiment,the setd7+/-mutant tilapia demonstrated stronger hypoxia tolerance,with the onset of surface swimming occurring significantly later than in the wild type,and a markedly extended survival time in low oxygen environments.This indicates that the mutation of the setd7 gene significantly enhances the hypoxia adaptation ability of tilapia.This study found that mutations in the methyltransferase gene setd7 can significantly improve the hypoxia tolerance of Nile tilapia,providing important experimental materials and theoretical foundations for molecular breeding of hypoxia tolerant tilapia.Additionally,this research lays a scientific foundation for further exploration of the molecular mechanisms and functional studies of methyl-transferases in hypoxia adaptation.关键词
尼罗罗非鱼/甲基转移酶基因/setd7基因/基因编辑/耐低氧Key words
Oreochromis niloticus/methyltransferase/setd7 gene/gene editing/hypoxia tolerance分类
水产学引用本文复制引用
何宏阳,王伊凡,贾海旺,周艳,陈良标,祖尧..setd7对尼罗罗非鱼的低氧耐受能力的影响[J].水产学报,2025,49(12):31-39,9.基金项目
国家重点研发计划(2024YFD2402400,2022YFD2400801) （2024YFD2402400,2022YFD2400801）
国家自然科学基金(32170423,31501166) National Key Research and Development Program of China(2024YFD2402400,2022YFD2400801) （32170423,31501166）
National Natural Science Foundation of China(32170423,31501166) （32170423,31501166）
