水产学报2025，Vol.49Issue(12)：40-50,11.DOI:10.11964/jfc.20250314925
低氧胁迫对斑石鲷能量代谢、肝脏抗氧化能力和组织形态变化的影响
Effects of hypoxic stress on the energy metabolism,hepatic antioxidant capacity and histomorphology in Oplegnathus punctatus
摘要
Abstract
Dissolved oxygen is a crucial environmental factor that significantly influences the survival,growth,and develop-ment of aquatic organisms.Fish species are diverse,and their physiological responses to hypoxic stress vary considerably.To investigate the impact of hypoxic stress on the antioxidant capacity,energy metabolism,and tissue morphology of Oplegnathus punctatus,a valuable species for deep-sea aquaculture,this study examined alterations in hepatic antioxidant enzyme activities,glycolipid metabolite levels,and tissue morphology in two size groups(200 g and 50 g)of O.punctatus under critical oxygen partial pressure(Pcrit)and loss of equilibrium(LOE)conditions.The results revealed that under hypoxic stress reaching LOE,the activities of lactate dehydrogenase(LDH),pyruvate kinase(PK),phosphofructokinase(PFK),and hexokinase(HK),as well as lactate content,significantly increased(P<0.05)in both size groups,with the 200 g group showing greater increases in energy metabolism enzyme activities compared to the 50 g group.Hepatic glycogen content decreased during hypoxia,with a significant reduction in the 50 g group at LOE(P<0.05),while the 200 g group showed no significant change.Concurrently,the activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)in the liver were significantly elevated(P<0.05),while malondialdehyde(MDA)content also increased significantly(P<0.05),reflecting heightened oxidative stress levels.Notably,the 50 g group exhibited smaller increases in antioxidant enzyme activities compared to the 200 g group.In contrast,the activities of lipoprotein lipase(LPL)and fatty acid synthase(FAS)showed no significant changes during hypoxia(P>0.05).Furthermore,the proportions of hepato-cyte migration,lysis,and pyknosis significantly increased in both size groups,whereas the proportion of normal hepatocytes gradually decreased.All measured parameters returned to control levels 24 hours after restoration of normal dissolved oxygen.In conclusion,hypoxic stress significantly affected the glycolysis,hepatic antioxidant capacity,and tissue morphology of O.punctatus in this experiment.The fish primarily relied on anaerobic glycolysis to meet energy demands under hypoxic condi-tions,with the 200 g group exhibiting more pronounced changes compared to the 50 g group.These results provide a theoret-ical foundation and data support for the effectively management of O.punctatusin captivity.关键词
斑石鲷/低氧胁迫/抗氧化能力/能量代谢/组织形态Key words
Oplegnathus punctatus/hypoxic stress/liver antioxidant capacity/energy metabolism/histomorphology分类
水产学引用本文复制引用
陈帅钰,高涛,刘志飞,汪峰,高云涛,陈夏添,贾玉东..低氧胁迫对斑石鲷能量代谢、肝脏抗氧化能力和组织形态变化的影响[J].水产学报,2025,49(12):40-50,11.基金项目
中国水产科学研究院基本科研业务费专项(CAFS 2023TD81,20603022025013) （CAFS 2023TD81,20603022025013）
山东省自然科学基金(ZR2025QC239) （ZR2025QC239）
青岛市博士后科学基金(QDBSH20240102012) Central Public-interest Scientific Institution Basal Research Fund(CAFS 2023TD81,20603022025013) （QDBSH20240102012）
Natural Science Foundation of Shandong Province(ZR2025QC239) （ZR2025QC239）
Qingdao Postdoctoral Science Foundation(QDBSH20240102012) （QDBSH20240102012）
