水产学报2025，Vol.49Issue(12)：103-112,10.DOI:10.11964/jfc.20240614564
天然水域人工模拟产卵环境诱导长江鲟自然繁殖后代的亲权分析
Parentage analysis of offspring induced by artificial simulated spawning environments in natural waters for natural breeding of the Acipenser dabryanus
摘要
Abstract
In 2023,a successful natural breeding experiment for the Acipenser dabryanus was conducted in an artificial simu-lated spawning environments within natural waters.To assess the effectiveness of this wild breeding experiment and to gain a deeper understanding of the breeding and mating patterns of Acipenser dabryanus,this study employed the mitochondrial D-loop region and 24 microsatellite for parentage assignment and genetic diversity analysis of parents and their offspring.The res-ults showed that among the 20 parental sturgeons,7 male and 7 female individuals participated in reproduction.Mitochondrial DNA analysis indicated that 240 offspring shared two identical mitochondrial haplotypes with 20 parents.Furthermore,microsatellite loci analysis revealed that these 240 offspring originated from 30 different parental combinations,with 223 off-spring successfully matched to their parental lineage,resulting in a high matching success rate of 92.92%.Genetic diversity analysis indicated that the 20 parental sturgeons exhibited a high level of genetic diversity,making them suitable as breeding stock.The genetic diversity of the offspring group was essentially consistent with that of the parents,indicating that the wild breeding experiment effectively maintained the genetic diversity to the offspring group.Three males contributed to 78.92%of offspring,while four females accounted for 91.48%of the offspring.These results demonstrate that A.dabryanus adopts a mixed mating strategy involving multiple females and males.This reproductive strategy is effective for the conservation genetic diversity within the natural population of A.dabryanus.This study not only provides valuable information for the genetic diversity and reproductive genetic management of A.dabryanus but also has significant guiding importance for future wild release and population recovery efforts.关键词
长江鲟/亲子鉴定/遗传多样性/交配模式/种群恢复Key words
Acipenser dabryanus/parentage analysis/genetic diversity/mating pattern/population recovery分类
水产学引用本文复制引用
程佩琳,刘焕章,杜浩,刘青娟,李鹏程,吴金明,李君轶,冷小茜,吴金平,仲嘉,俞丹..天然水域人工模拟产卵环境诱导长江鲟自然繁殖后代的亲权分析[J].水产学报,2025,49(12):103-112,10.基金项目
国家重点研发计划(2021YFD1200304) （2021YFD1200304）
中国水产科学研究院中央级公益性科研院所基本科研业务费(2023TD08) （2023TD08）
国家自然科学基金(32303009) National Key Research and Development Program of China(2021YFD1200304) （32303009）
Central Public-interest Scientific Institution Basal Research Fund,CAFS(2023TD08) （2023TD08）
National Natural Science Foundation of China(32303009) （32303009）
