水产学报2025，Vol.49Issue(12)：113-129,17.DOI:10.11964/jfc.20230413976
异速生长的七彩神仙鱼生长、碳氮转化和转录组学分析
Growth,carbon and nitrogen balances,and transcriptome analysis of the allometric growth in Symphysodon haraldi
摘要
Abstract
To explore the potential process of growth heterogeneity in discus fish,this experiment selected five pairs of the same species of discus fish as parents and cultivated their offspring for 60 days.The offspring were divided into two extreme groups with significant differences in body weight:the slow-growing group(S group)with the weight of(0.153±0.011)g and the fast-growing group(F group)with the weight of(0.347±0.030)g.After 30 days of cultivation,the growth,nutrient diges-tion,carbon and nitrogen balance,and transcriptome analysis of the visceral tissue of the discus fish were measured.The res-ults showed that,in terms of growth,the average daily weight gain,average daily feed intake and feed efficiency of the F group were significantly higher than those of the S group.In terms of digestion,the apparent digestibility of dry matter,carbon,and nitrogen in the F group was significantly higher than those in the S group;the activities of α-amylase and gastric protease in the viscera of the F group were significantly higher than those in the S group.In terms of carbon and nitrogen balance,the F group absorbed more carbon and nitrogen,mainly for growth,and excreted less carbon and nitrogen through feces and respiration/excretion than the S group.Transcriptome analysis showed that the differentially expressed genes between the two groups were mainly enriched in signal pathways such as myocardial contraction,calcium signaling,glycolysis/gluconeogenesis,PPAR,thyroid hormone,purine metabolism,insulin secretion,galactose metabolism,and the pentose phosphate pathway.Among them,the significantly upregulated genes in the F group included ann,mid1,pur,fat,elo,acy,and dcK,and both the PPAR signaling pathway and purine metabolism may be related to the rapid growth of the F group.The results indicate that compared to the small-sized discus fish,the large-sized individuals have stronger growth and digestion abilities and higher util-ization of carbon and nitrogen,which may be related to the activation of the PPAR signaling pathway and purine metabolism,providing a theoretical basis for the nutritional intervention and breeding of allometric growth fish.关键词
七彩神仙鱼/异速生长/消化/碳氮转化/转录组Key words
Symphysodon haraldi/allometric growth/digestion/carbon and nitrogen balances/transcriptome分类
水产学引用本文复制引用
邹奥锟,高建忠,温彬,陈再忠..异速生长的七彩神仙鱼生长、碳氮转化和转录组学分析[J].水产学报,2025,49(12):113-129,17.基金项目
上海市自然科学基金(20ZR1423600) （20ZR1423600）
上海扬帆人才计划(19YF1419400) Shanghai Natural Science Foundation(20ZR1423600) （19YF1419400）
Shanghai Sailing Program(19YF1419400) （19YF1419400）
