水产学报2025，Vol.49Issue(12)：130-148,19.DOI:10.11964/jfc.20241014718
养殖密度对斑节对虾生长、肝胰腺抗氧化、免疫性能及其相关基因表达和肠道健康的影响
Effects of stocking density on growth,hepatopancreatic antioxidant,immune performance,related gene expression,and intestinal health of Penaeus monodon
摘要
Abstract
The study was carried out to investigate the optimal stocking density of Penaeus monodon.Healthy shrimps[initial body weight(2.55±0.19)g,body length(6.71±0.20)cm]were randomly distributed into six different stocking densities(225,300,375,450,525,and 600 ind/m3),labeled as D225,D300,D375,D450,D525,and D600,respectively.Culturing for 42 days.The results showed that the survival rate(SR)gradually decreased with increasing stocking density.Furthermore,the weight gain rate(WGR),specific growth rate(SGR),and condition factor(CF)were increased first and then decreased,while the feed conversion ratio(FCR)was conversely.Antioxidant and immune properties of hepatopancreatic indicated that D375 showed the best antioxidant and immune performance,whose activities of SOD,AKP,and ACP were significantly higher than that of the other groups(P<0.05),and the MDA content and expression of Prx,p53,MyD88,and LZM were significantly lower than that of the other groups(P<0.05).Regarding intestinal health,the Alpha diversity index of intestinal microbiota showed a trend to increase first and then decrease with the increasing stocking density.The Alpha diversity of D225 and D600 was signific-antly lower than that of the other groups(P<0.05).As for species distribution of intestinal microbiota,D450 showed the highest relative abundance of beneficial bacteria(e.g.,Firmicutes and Ligilactobacillus)and the lowest one of harmful bacteria(e.g.,Proteobacteria).By observing the intestinal structure and morphology,it was found that the intestinal structure of D375 and D450 was more complete,the villi arranged densely and neatly,and the villi height was higher than that of the other groups.In contrast,the other groups exhibited partial epithelial cell sloughing and villus loss.In summary,the study indicates that excess-ively high or low stocking density was not conducive to shrimp growth and physiological function regulation.The stocking density of 375-450 ind/m3 promoted growth,improved hepatopancreas antioxidant capacity and non-specific immunity and optimized the intestinal microbial community structure of the shrimp.This study provided an experimental basis for the optimum culturing density of P.monodon,which could help to improve the culture efficiency and promote the development of sustainable and healthy shrimp aquaculture.关键词
斑节对虾/养殖密度/抗氧化/免疫/肠道健康Key words
Penaeus monodon/stocking density/antioxidant/immunity/intestinal health分类
农业科技引用本文复制引用
俞韩绣,唐海南,许金震,李金鑫,刘智武,黄永春,蔡章印..养殖密度对斑节对虾生长、肝胰腺抗氧化、免疫性能及其相关基因表达和肠道健康的影响[J].水产学报,2025,49(12):130-148,19.基金项目
福建省高校产学合作项目(2023N5007) （2023N5007）
福建省科技特派员后补助项目(2021S2001) （2021S2001）
福建省海洋服务与渔业高质量发展专项(FJHY-YYKJ-2022-3-3) Fujian Provincial University-industry Cooperation Project(2023N5007) （FJHY-YYKJ-2022-3-3）
Fujian Provincial Science and Technology Specialist Post-supplement Project(2021S2001) （2021S2001）
Fujian Provincial Special Fund Project for High-quality Develop-ment of Marine Services and Fisheries(FJHY-YYKJ-2022-3-3) （FJHY-YYKJ-2022-3-3）
