四川中医2025，Vol.43Issue(10)：179-186,8.DOI:10.26946/j.cnki.1000-3649.sczy.2503230001
活血化瘀合剂对兔膝骨关节炎模型TGF-β1/Smad信号通路干预作用
Effect of the blood-activating and stasis-resolving mixture on the TGF-β1/Smad signaling pathway in rabbit knee osteoarthritis model
摘要
Abstract
Objective To investigate the regulatory effect of the blood-activating and stasis-resolving mixture on the TGF-β1/Smad signaling pathway in a rabbit model of knee osteoarthritis(KOA).Methods Thirty New Zealand rabbits were randomly divided into blank group,sham operation group,model group,Chinese herbal decoction group,and positive drug group.After successfully modelling by modified Hulth method,the rabbits were given gavage according to the groups for 4 weeks.4 weeks later,knee cartilage of rabbits was taken to observe the injury degree of rabbit articular cartilage,and the expression levels of PPAR,PARP1,TGF-β1,Smad2/3 and p-Smad2/3 proteins were detected.Results The model group exhibited significant articular cartilage damage,with Mankin scores significantly higher than those of the blank control and sham-operated groups(P<0.01).Expression levels of PPAR and PARP1 were upregulated,while protein levels of TGF-β1 and p-Smad2/3 were downregulated.Additionally,mRNA expression of TGF-β1,p-Smad2/3,and Col1A1 was reduced(P<0.01).In contrast,both the Chinese herbal compound group and the positive drug group showed significantly lower Mankin scores than the model group(P<0.01).Protein expression levels of TGF-β1 and p-Smad2/3 were higher than those in the model group,and mRNA expression of TGF-β1,p-Smad2/3,and Col1A1 was also elevated compared to the model group(P<0.01).Conclusion The blood-activating and stasis-resolving mixture can protect the articular cartilage and exert an intervention effect on KOA by regulating the TGF-β1/Smad signaling pathway in the rabbit knee osteoarthritis model and increasing the levels of proteins related to the TGF-β1/Smad signaling pathway.关键词
膝骨关节炎/TGF-β1/Smad/活血化瘀合剂/关节软骨Key words
Knee osteoarthritis/TGF-β1/Smad/The blood-activating and stasis-resolving mixture/Articular cartilage分类
医药卫生引用本文复制引用
熊逸啸,白林寒,汤祖宇,李艾佳,肖珲玥,沈诚苇,欧梁,余毅..活血化瘀合剂对兔膝骨关节炎模型TGF-β1/Smad信号通路干预作用[J].四川中医,2025,43(10):179-186,8.基金项目
湖南中医药大学校级课题(2022FTKFJJ13). （2022FTKFJJ13）
