陕西中医2025，Vol.46Issue(11)：1443-1448,6.DOI:10.3969/j.issn.1000-7369.2025.11.001
整体调节针法对骨质疏松大鼠骨代谢及Th17/Treg失衡的作用机制
Regulation of bone metabolism and Th17/Treg imbalance in osteoporotic rats by overall adjustment acupuncture
摘要
Abstract
Objective:In the context of Th17/Treg cell balance,this study aimed to explore impact of the holistic regulatory acupuncture therapy on rats with osteoporosis(OP).Methods:30 rats were randomly and equally divided into three groups:the sham-operated group,the model group,and the holistic regulatory acupuncture group.The OP model was established through bilateral ovariectomy.In the holistic regulatory acupuncture group,rats received acu-puncture at Zusanli,Shenshu,and Dazhu.Additionally,mild moxibustion treatment was administered at bilateral Shenshu for a duration of three months.Upon completion of the intervention,Micro-CT was employed to assess the morphological parameters of the rat femur,including bone mineral density(BMD),bone volume fraction(BV/TV),trabecular number(Tb.N),trabecular separation(Tb.Sp),and trabecular thickness(Tb.Th).Flow cytometry was utilized to quantitatively analyze the proportions of Th17 and Treg cells in the spleen.Enzyme-linked immunosorbent assay(ELISA)was used to measure the levels of alkaline phosphatase(ALP),type Ⅰ procollagen N-terminal propeptide(PⅠ NP),tartrate-resistant acid phosphatase 5b(TRACP-5b),and type Ⅰ collagen C-terminal telopep-tide(CTX-Ⅰ)in the serum.Results:Compared with the sham-operated group,the model group exhibited a decrease in BMD,BV/TV,Tb.N,and Tb.Th,along with an increase in Tb.Sp(all P＜0.05).Regarding bone metabolism,the levels of ALP and P Ⅰ NP decreased,while those of TRACP-5b and CTX-Ⅰ increased(all P＜0.05).Moreover,in the model group,the proportion of Th17 cells and the Th17/Treg ratio increased,while the proportion of Treg cells de-creased(P＜0.05).In contrast,the holistic regulatory acupuncture group significantly reversed these abnormal indi-cators compared to the model group(P＜0.05).Conclusion:The holistic regulatory acupuncture therapy exerts an anti-OP effect by improving bone microstructure,regulating bone metabolism markers,and rectifying the Th17/Treg imbalance.关键词
骨质疏松/整体调节针法/骨微结构/骨代谢/Th17/Treg平衡/免疫失衡Key words
Osteoporosis/Overall adjustment acupuncture/Bone microstructure/Bone metabolism/Th17/Treg balance/Immune imbalance分类
中医学引用本文复制引用
黄梅,李莉,吴柯楠,王祖红..整体调节针法对骨质疏松大鼠骨代谢及Th17/Treg失衡的作用机制[J].陕西中医,2025,46(11):1443-1448,6.基金项目
国家自然科学基金资助项目(82360977) （82360977）
云南省科技厅科技计划项目(202301AZ070001-151) （202301AZ070001-151）
