陕西中医2025，Vol.46Issue(11)：1462-1468,7.DOI:10.3969/j.issn.1000-7369.2025.11.004
基于内皮功能调控探讨培元降压方干预大鼠高血压模型作用机制
Mechanism of Peiyuan Jiangya formula in intervening hypertensive model rats based on endothelial function regulation
摘要
Abstract
Objective:This study aimed to investigate antihypertensive effect of Peiyuan Jiangya formula onL-NAME-induced hypertensive rat models and its protective mechanism on vascular endothelial function.Methods:32 SD rats were randomly divided into four groups:blank group,model group,Peiyuan Jiangya formula group and Tianma Gouteng decoction group,with 8 rats in each group.Except for the blank group,the other groups were intra-gastrically administered L-NAME at 50 mg/(kg·d)for 4 consecutive weeks to establish the hypertensive model.After successful modeling,the corresponding drugs were administered for 8 weeks.During experiment,the general condition and blood pressure of the rats were monitored.After the intervention,vascular dilation function was evalua-ted by isolated vascular rings.The morphology of mesenteric artery tissue was observed by HE staining.The levels of serum nitric oxide(NO),vascular endothelial growth factor(VEGF),and endothelin-1(ET-1)were detected by ELISA.The content of reactive oxygen species(ROS)was detected by fluorescence detection.The expression of en-dothelial nitric oxide synthase(eNOS)and its phosphorylation(p-eNOS)in aortic tissue was detected by Western blot.Results:After 8 weeks of treatment,compared with the model group,the Peiyuan Jiangya formula group signifi-cantly reduced blood pressure in hypertensive rats(all P＜0.01),enhanced endothelium-dependent vasodilation,im-proved vascular endothelial injury,increased serum NO and VEGF levels,decreased ET-1 levels,reduced the genera-tion of reactive oxygen species in tissues,and promoted the expression of eNOS and p-eNOS in aortic tissue,differ-ences statistically significant(all P＜0.01).Conclusion:Peiyuan Jiangya formula has certain antihypertensive effect on L-NAME-induced hypertensive rats and can effectively improve vascular endothelial function.It exerts antihyper-tensive and vascular protective effects by regulating the NO/ET-1 balance,reducing oxidative stress levels,and acti-vating eNOS/NO signaling pathway.关键词
高血压/培元降压方/血管内皮损伤/一氧化氮/氧化应激/内皮型一氧化氮合酶/一氧化氮通路/一氧化氮合酶抑制模型Key words
Hypertension/Peiyuan Jiangya formula/Vascular endothelial dysfunction/Nitric oxide/Oxidative stress/Endothelial nitric oxide synthase/Nitric oxide pathway/Nitric oxide synthase inhibition model分类
医药卫生引用本文复制引用
刘韵琳,刘可欣,刘学,冯银,范小璇..基于内皮功能调控探讨培元降压方干预大鼠高血压模型作用机制[J].陕西中医,2025,46(11):1462-1468,7.基金项目
国家自然科学基金资助项目(82374548) （82374548）
国家中医药管理局高水平中医药重点学科建设项目(国中医药人教函[2022]226号) （国中医药人教函[2022]226号）
陕西省中医脑病临床医学研究中心项目(陕科社发[2017]176号) （陕科社发[2017]176号）
陕西省血管老化和神经退行性疾病中西医防治创新团队项目(2022-SLRH-LJ-015) （2022-SLRH-LJ-015）
