陕西中医2025，Vol.46Issue(11)：1476-1480,1519,6.DOI:10.3969/j.issn.1000-7369.2025.11.006
孙氏头腹针刺法对焦虑性失眠大鼠BDNF-TrkB信号通路的影响
Mechanism of Sun's head-abdominal acupuncture on anxiety-induced insomnia rats via the BDNF-TrkB signaling pathway
摘要
Abstract
Objective:To observe effect of Sun's head-abdominal acupuncture on the BDNF-TrkB signaling pathway expression in rats with anxiety-induced insomnia.Methods:SD rats were randomly divided into control group,model group,conventional acupuncture group,and head-abdominal acupuncture group.Except for the control group,anxiety-insomnia models were established in the other three groups using sleep deprivation,anxiety-inducing psychological stress,and intraperitoneal injection of para-chlorophenylalanine.The conventional acupuncture group received standard acupoint treatment,while the head-abdominal acupuncture group received combined head and ab-dominal acupuncture with electroacupuncture(2 Hz continuous wave,0.5 mA)applied to head acupoints,30 minutes daily over one week.Behavioral assessments,including the pentobarbital-induced sleep test and open field test(OFT),were conducted.Additionally,Western blot(WB)and quantitative polymerase chain reaction(qPCR)were employed to detect hippocampal BDNF-TrkB mRNA and protein expression levels.Results:Compared to the control group,the model group exhibited prolonged sleep latency(SL),shortened sleep duration(ST)(P＜0.01),and re-duced horizontal/vertical movement in OFT(all P＜0.05),confirming successful modeling.Both acupuncture groups showed shortened SL,prolonged ST(P＜0.01),and increased motor activity(P＜0.05)compared to the model group.The head-abdominal acupuncture group further outperformed the conventional group in SL reduction and ver-tical exploration(P＜0.05).Compared with the control group,the model group showed significantly decreased mR-NA and protein expression of BDNF and TrkB(P＜0.01).Both acupuncture groups exhibited upregulated BDNF-TrkB expression(P＜0.05),with the head-abdominal group showing higher BDNF and TrkB protein levels than the conventional group(P＜0.05).Conclusion:Both Sun's head-abdominal acupuncture and conventional acupuncture can improve sleep quality,alleviate anxiety-like behaviors,and enhance BDNF-TrkB signaling in anxiety-insomnia rats.Notably,Sun's head-abdominal acupuncture shows superior therapeutic efficacy,providing a novel clinical treat-ment strategy for anxiety-induced insomnia.关键词
焦虑性失眠/孙氏头腹针/脑源性神经营养因子/酪氨酸激酶受体B/戊巴比妥钠协同睡眠实验Key words
Anxiety insomnia/Sun's head-abdominal acupuncture/BDNF/TrkB/Pentobarbital-induced sleep test分类
中医学引用本文复制引用
覃颖,李笑涵,吴江滨,秦丽娜..孙氏头腹针刺法对焦虑性失眠大鼠BDNF-TrkB信号通路的影响[J].陕西中医,2025,46(11):1476-1480,1519,6.基金项目
国家重点研发计划"常见多发病防治研究"重点专项课题(2022YFC2503900) （2022YFC2503900）
北京中医药大学新教师启动基金资助项目(2023-JYB-XJSJJ058) （2023-JYB-XJSJJ058）
