生物安全学报（中英文）2025，Vol.34Issue(4)：338-344,7.DOI:10.3969/j.issn.2095-1787.20240095
进境法国大麦中麦类壳多胞斑点病菌的鉴定及致病性测定
Identification and pathogenicity evaluation of Parastagonospora pseudonodorum in barley imported from French
摘要
Abstract
[Aim]This study aims to identify Parastagonospora pseudonodorum in barley imported from French and evaluate its pathogenicity.[Method]A fungal isolate 5225-17 was obtained from the barley sample imported from French by the plate culture method,and it was identified based on colony morphology as well as ITS,β-tubulin,and β-xylosidase sequences.Furthermore,the pathogenicity of this isolate was tested.[Result]The colony of 5225-17 cultured on potato dextrose agar(PDA)appeared white flocculent in the front view and light pink in the center in the back view,with a regular margin.The isolate experienced rapid growth at a rate of 0.5～1.0 cm·d-1.It showed low sporulation on PDA and sporulation on water agar after 21 days of incubation at 20℃.The conidia were cylindrical,transparent,slightly curved or straight at the upper end,blunt or round at the end,with 3～5 septa,oil balls,and the average size of 26.8 μm×3.6 μm.The asci were rod-shaped,transparent,with a round top,(40～80)μm×(8～11)μm.Each ascus contained 8 ascospores,being light yellow to light yellowish brown,spindle-shaped,straight or slightly curved,obviously constricted at septa,with 3 septa,occasionally 2 or 4,and average size of 20.8 μm×4.1 μm.The ITS,β-tubulin,and β-xylosidasesequences of the isolate were identical to those of Parastagonospora pseudonodorum.The phylogenetic tree built based on multiple genes indicated that isolate 5225-17 shared the same clade with P.pseudonodorum and had a distant genetic relationship with P.nodorum.The pathogenicity test showed that isolate 5225-17 caused typical symptoms on both detached and living leaves.[Conclusion]The isolate 5225-17 is identified as P.pseudonodorum.关键词
麦类壳多胞斑点病菌/大麦/分离鉴定/致病性Key words
Parastagonospora pseudonodorum/barley/isolation and identification/pathogenicity引用本文复制引用
叶露飞,牟桂萍,闫晓东,杨赛军,左然玲,易建平..进境法国大麦中麦类壳多胞斑点病菌的鉴定及致病性测定[J].生物安全学报（中英文）,2025,34(4):338-344,7.基金项目
广东省口岸安全智能化检测重点实验室项目(2023B1212010011) （2023B1212010011）
2023年度促进经济高质量发展专项资金(实施标准化战略) （实施标准化战略）
"十四五"广东省农业科技创新十大主攻方向项目(2022SDZG06) （2022SDZG06）
