生物安全学报（中英文）2025，Vol.34Issue(4)：345-352,8.DOI:10.3969/j.issn.2095-1787.20240130
引起水稻细菌性穗枯病的Burkholderia glumae的分离鉴定
Isolation and identification of Burkholderia glumae causing bacterial panicle blight of rice
摘要
Abstract
[Aim]The pathogen causing bacterial panicle blight of rice in Xinan Village,Baizhang Town,Minqing County,Fujian Province and its biological characteristics are studied,with the aim of providing guidance for the prevention and control of this dis-ease.[Method]The tissue culture method was employed to isolate the pathogen,the pathogenicity of which was then verified by Koch's postulates.The morphological characteristics,physiological and biochemical properties,and molecular evidence were used to identify the pathogen.[Result]Seven bacterial strains were isolated from rice samples,which were confirmed by Koch's postulates to be the pathogens of bacterial panicle blight.The bacterial strains were gram-negative,forming round and light yellow to earth yellow colonies with smooth edges on NA,KB,PDA,and CCNT plates.The strains showed optimum growth at 34℃and pH7.0.The physiological and biochemical test results of the strains were consistent with those of the control strain Burkholderia glumae GX16.A single target band was obtained by amplification of Bglu3F/R,specific primers of B.glumae.Based on the results above and 16S rDNA,gyrB,and rpoD sequences,the strains were identified as B.glumae.[Conclusion]The pathogenic bacteria causing bacterial panicle blight of rice in Xinan Village,Baizhang Town,Minqing County,Fujian Province is B.glumae.关键词
水稻细菌性穗枯病/颖壳伯克氏菌/生物学特性/gyrB/rpoDKey words
bacterial panicle blight of rice/Burkholderia glumae/biological characteristics/gyrB/rpoD引用本文复制引用
谢雪金,张一帆,刘国坤,蔡学清..引起水稻细菌性穗枯病的Burkholderia glumae的分离鉴定[J].生物安全学报（中英文）,2025,34(4):345-352,8.基金项目
福建省科技重大专项(2022NZ0106) （2022NZ0106）
中国烟草总公司科技计划项目[110202201028(LS-12)] （LS-12）
福建现代农业(水稻)产业技术体系建设项目(闽财教指[2019]144号) （水稻）
