生物安全学报（中英文）2025，Vol.34Issue(4)：399-405,7.DOI:10.3969/j.issn.2095-1787.20240142
南方根结线虫长链非编码RNA的鉴定与分析
Identification and analysis of Meloidogyne incognita lncRNAs
摘要
Abstract
[Aim]Root-knot nematodes(Meloidogyne spp.)can parasitize a wide range of economically important crops,causing significant annual agricultural economic losses in our country.Identifying and analyzing long non-coding RNAs(lncRNAs)in root-knot nematodes can lay the foundation for a deeper understanding of their gene expression regulation and parasitism mechanisms.[Method]High-throughput sequencing and bioinformatics analysis were employed to identify lncRNAs in Meloidogyne incognita at pre-parasitic J2 stage,parasitic J3/J4 stages,and adult female stage.Comparative analysis between the pre-parasitic and parasitic stages were conducted to determine the expression patterns of lncRNAs in M.incognita.Additionally,potential target genes of lncR-NAs were predicted and subjected to functional enrichment analysis.[Result]A total of 655 lncRNAs were identified in M.incogni-ta,with lengths primarily ranging from 200-1 000 nucleotides.The GC content of these lncRNAs was significantly lower than that of protein-coding genes.Differential expression analysis revealed 254 and 251 differentially expressed lncRNAs in the J3/J4 and adult female stages,respectively,compared to the pre-parasitic J2 stage.Target gene prediction identified 354 and 372 differentially ex-pressed target genes in the J3/J4 and adult female stages,respectively.KEGG enrichment analysis of these differentially expressed target genes highlighted their involvement in processes such as the basal transcription factors,spliceosome,nucleocytoplasmic trans-port,and mRNA surveillance pathway.[Conclusion]This study provides crucial insights into the lncRNA landscape in M.incogni-ta,elucidating their expression patterns potential functional associations with target genes.These findings lay a foundation for further investigation into the gene expression regulation and parasitic mechanisms of the M.incognita.关键词
南方根结线虫/非编码RNA/基因表达调控Key words
Meloidogyne incognita/lncRNAs/gene expression regulation引用本文复制引用
朱召禄,万人静,彭海,宋会银,李甜甜,高利芬,肖紫兰,李论,方治伟,周俊飞,陈利红..南方根结线虫长链非编码RNA的鉴定与分析[J].生物安全学报（中英文）,2025,34(4):399-405,7.基金项目
江汉大学一流学科建设重大专项资助计划(2023XKZ024) （2023XKZ024）
江汉大学校级项目(2023KJZX44、2023KJZX40) （2023KJZX44、2023KJZX40）
