生物加工过程2025，Vol.23Issue(5)：483-490,500,9.DOI:10.3969/j.issn.1672-3678.2025.05.002
重组枯草芽孢杆菌转化生成活性维生素D3的发酵工艺优化
Optimization of fermentation process for the recombinant Bacillus subtilis to synthesize 25(OH)VD3
摘要
Abstract
25-Hydroxyvitamin D3(25(OH)VD3),as one of the main active forms of vitamin D3(VD3)in human body,provides high medicinal values.A recombinant strain WB600-pMA5-CYP109E1I85F-Fdx-FdR,which was previously constructed in our laboratory,could selectively hydroxylate C-25 of VD3 to produce 25(OH)VD3.Herein,our study was focused on further optimization of the fermentation process with this recombinant strain to convert VD3 to 25(OH)VD3.First,the types and concentrations of carbon sources,nitrogen sources,and metal ions in the culture medium were screened through single factor experiments and orthogonal experiments.As a result,the optimal medium components were peptone 50 g/L,yeast powder 50 g/L,glucose 30 g/L,and dipotassium hydrogen phosphate 9 g/L.Under the optimized conditions,the fermentation with this recombinant strain could convert 0.25 g/L VD3 to 10.01 mg/L 25(OH)VD3,which was 5.5 times more efficient than previous process.Then,the types and concentrations of cosolvents were investigated for such transformation to 25(OH)VD3.Delightfully,the addition of 3％(V/V)of DMSO to the medium could generate much higher concentration of product 25(OH)VD3(17.74 mg/L).Our research work demonstrated that optimization of the fermentation process with the recombinant strain could significantly increase the production of 25(OH)VD3.Further investigation of this transformation can be conducted at the fermentation tank level to improve the synthesis of 25(OH)VD3,presenting the great significance for the industrial application.关键词
25(OH)VD3/枯草芽孢杆菌/微生物转化法/发酵过程优化/VD3羟基化酶Key words
25(OH)VD3/Bacillus subtilis/microbial transformation method/optimization of fermentation process/hydroxylase of VD3分类
生物工程引用本文复制引用
勾钧恒,何凡,麻巧巧,何于琳,史劲松,李会..重组枯草芽孢杆菌转化生成活性维生素D3的发酵工艺优化[J].生物加工过程,2025,23(5):483-490,500,9.基金项目
中国电子系统工程第二建设有限公司横向课题(231825、241336) （231825、241336）
