生物加工过程2025，Vol.23Issue(5)：521-529,9.DOI:10.3969/j.issn.1672-3678.2025.05.006
联苯降解菌YS-33胞外多糖的生物活性与流变特性研究
Biological activity and rheological properties of extracellular polysaccharides from Dyella ginsengisoli YS-33
摘要
Abstract
YS-33,a strain to degrade polycyclic aromatic hydrocarbon,was isolated from the soil of a coking plant.Through physiological,biochemical,and 16S rRNA sequencing,this strain was identified to be the biphenyl degrading bacterium(Dyella ginsengsoli YS-33).The extracellular polysaccharide(DGPS)of the bacterium were obtained by fermentation,which was further analyzed by high performance liquid chromatography(HPLC)and high performance gel permeation chromatography(HPGPC).As a result,DGPS proved to be an acidic polysaccharide composed of glucuronic acid(GlcAc)and galactose(Gal),with a molar ratio of 1∶6.8,and the molecular weight of DGPS was determined to be 1.71×106.Importantly,polysaccharides displayed good antioxidant activity.Based on the subsequent rheological research,DGPS solution exhibited non-Newtonian fluid shear thinning characteristics,with its apparent viscosity in concentration-and temperature-dependent manner.Moreover,the viscosity of DGPS could be affected by the type and amount of metal ions added,with the apparent viscosity reaching its maximum under high concentration,low salt,and neutral pH conditions.Overall,DGPS demonstrated good antioxidant activity and temperature stability,and has potential applications in the fields of daily chemical and environmental protection.关键词
Dyella ginsengisoli/微生物多糖/抗氧化活性/流变学特性Key words
Dyella ginsengisoli/microbial polysaccharides/antioxidant activity/rheological properties分类
生物科学引用本文复制引用
杜玮,孟巧,何跃,章雷,张可桂,刘迎..联苯降解菌YS-33胞外多糖的生物活性与流变特性研究[J].生物加工过程,2025,23(5):521-529,9.基金项目
生态环境部南京环境科学研究所基本科研业务专项(GYZX220302) （GYZX220302）
