生物加工过程2025，Vol.23Issue(5)：530-537,8.DOI:10.3969/j.issn.1672-3678.2025.05.007
产脲酶细菌NS-6和大豆脲酶诱导CaCO3沉淀的研究
Studies on calcium carbonate precipitation induced by urease-producing bacterium NS-6 and soybean urease
李建山 1杨立安 1鄢长灏 1闫若勤 1屈肖 1徐瑞 2马艳玲2
作者信息
- 1. 中国石油长庆油田分公司 油气工艺研究院,陕西西安 710018
- 2. 西北大学生命科学学院,陕西西安 710069
- 折叠
摘要
Abstract
Urease-producing bacterial Neobacillus mesonae NS-6 and soybean urease were evaluated with regards to their urease activities,as well as the yields and unconfined compressive strength of calcium carbonate(CaCO3)produced through the induction under different pH conditions.Meanwhile,the microstructural features of CaCO3 deposition,along with its cementing effects on quartz sand,were also examined in this study.The urease activity of strain NS-6 increased with incubation time and was stabilized at 7.9 mmol/(L·min)after 32 hours,while the urease activity from the soybean kept at a constant level of 6.0 mmol/(L·min).During the variation of pH values from 4 to 12,the cementation process experienced the initial growth followed by gradual decrease.In the end,the CaCO3 content and compressive strength by the urease-producing bacteria(12.9％and 0.239 MPa,respectively)were both higher than those of the soybean urease group(10.7％and 0.215 MPa,respectively).With the combination of SEM-EDS and XRD analyses,the CaCO3 precipitation induced by strain NS-6 was comprised of both calcite and vaterite,whereas the soybean urease mediated process exclusively gave calcite for the CaCO3.Furthermore,excellent cementation effect could be achieved by filling in the spaces between the quartz sand particles with CaCO3 crystals formed through both types of inductions.关键词
新型芽孢杆菌/大豆脲酶/CaCO3/抗压强度/微观结构Key words
Neobacillus mesonae/soybean urease/calcium carbonate/compressive strength/microstructure分类
生物学引用本文复制引用
李建山,杨立安,鄢长灏,闫若勤,屈肖,徐瑞,马艳玲..产脲酶细菌NS-6和大豆脲酶诱导CaCO3沉淀的研究[J].生物加工过程,2025,23(5):530-537,8.
