生物加工过程2025，Vol.23Issue(5)：538-545,8.DOI:10.3969/j.issn.1672-3678.2025.05.008
响应面法优化葛根茶多酚提取及其抗氧化活性研究
Optimization of extraction process and antioxidant activity of polyphenols from Pueraria lobata tea by response surface methodology
摘要
Abstract
The experiment is to optimize the extraction process of Pueraria lobata tea polyphenols and determine the antioxidant activity of resulting polyphenols.With Pueraria lobata tea as the raw material,the polyphenol substances in the tea were extracted by using ethanol solvent,and several factors including ethanol volume fraction,extraction time,extraction temperature and liquid-solid ratio on the polyphenol extraction amount,were optimized using the Box-Benhnken center combination test in response surface methodology.Meantime,the antioxidant activity of polyphenols extracted from Pueraria lobata tea was evaluated using DPPH radical(DPPH·)and hydroxyl radical(·OH)methods,and the scavenging abilities of polyphenols and Vc were compared in this case.As a result,the extraction amount of polyphenols could reach(14.87±0.39)mg/g under the optimal extraction conditions when the volume fraction of ethanol was 70％,the extraction time was 40 min,the extraction temperature was 60℃,and the ratio of liquid to solid was 80∶1 mL/g.The semi-inhibitory concentrations(IC50)of polyphenols extracted from Pueraria lobata tea on DPPH·radical and hydroxyl radical(·OH)were 96.21 μg/mL and 297.59 μg/mL,respectively.When the concentration of polyphenols was 100 μg/mL,the maximum scavenging rates of sample and Vc on DPPH·free radical were 51.33％±1.27％and 83.54％±2.28％,respectively.In addition,when the concentration was 200 μg/mL,the maximum scavenging rates of sample and Vc on·OH free radical were 46.10％±1.87％and 61.50％±1.75％,respectively.In summary,the polyphenols from Pueraria lobata tea demonstrated good antioxidant capacity.关键词
葛根茶/多酚/响应面法/提取工艺/抗氧化活性Key words
Pueraria lobata tea/polyphenols/response surface methods/extraction process/antioxidant activity分类
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莫周美,吴玲玲,李恒锐,郭素云,陈会鲜,林嘉雯,蔡兆琴,张秀芬..响应面法优化葛根茶多酚提取及其抗氧化活性研究[J].生物加工过程,2025,23(5):538-545,8.基金项目
广西农业科学院稳定资助科研团队(桂农科2021YT157) （桂农科2021YT157）
