生物加工过程2025，Vol.23Issue(5)：555-567,13.DOI:10.3969/j.issn.1672-3678.2025.05.010
响应面法优化液态发酵培养基和发酵条件促进间型脉胞菌OK菌丝体蛋白生产
Optimization of liquid fermentation medium and fermentation conditions for promoting the production of mycelial protein of Neurospora intermedia OK by response surface methodology
摘要
Abstract
To obtain the mycelial protein of Neurospora intermedia OK rapidly and efficiently,we optimized the corresponding medium composition and culture conditions for liquid fermentation through single-factor experiments and response surface methodology.As a result,the optimal medium components for mycelial protein were composed of sucrose(34 g/L),yeast extract(5 g/L),MgSO4(1.5 g/L),and vitamin B2(0.1 g/L).The optimal fermentation conditions were initial fermentation at pH 6,fermentation time for 5 days,fermentation temperature at 28℃,and fermentation rotation speed at 180 r/min.Through the improvement in this study,the mycelial protein yield could reach 3.64 g/L,which was 230.91％higher than the protein yield from the original basic medium.关键词
间型脉胞菌/液态发酵/培养基优化/培养条件优化/响应面优化Key words
Neurospora intermedia OK/liquid fermentation/medium optimization/culture condition optimization/response surface optimization分类
轻工业引用本文复制引用
韩思雨,戴意强,王喆,何子茵,王道营,孙玲,印伯星,夏秀东..响应面法优化液态发酵培养基和发酵条件促进间型脉胞菌OK菌丝体蛋白生产[J].生物加工过程,2025,23(5):555-567,13.基金项目
国家重点研发计划(2024YFF1106800) （2024YFF1106800）
