生物加工过程2025，Vol.23Issue(5)：568-574,7.DOI:10.3969/j.issn.1672-3678.2025.05.011
基于亲水相互作用色谱柱的超高效液相色谱串联质谱法同时检测葡萄中3种有机酸
Simultaneous determination of three organic acids in grapes by UPLC-MS/MS method based on HILIC column
摘要
Abstract
A high-sensitivity method was established for the simultaneous detection of citric acid,malic acid,and tartaric acid in grapes.Using the tailing factor as the core evaluation index,we systematically evaluated the separation performance of five chromatographic columns with different separation mechanisms,namely C18,T3,Amide,Diamond Hydride,and HILIC columns.As a result,the HILIC column,by combination with a 0.6％formic acid aqueous solution-a 0.6％formic acid-acetonitrile isocratic elution system,could effectively provide symmetric peak(tailing factor:0.95-1.05)by suppressing silanol ionization and enhancing hydrophilic interactions.Such a UPLC-MS/MS method demonstrated the following:detection limits(LODs)of 0.028-0.160 mg/L,quantification limits(LOQs)of 0.093-0.530 mg/L,spiked recoveries of 81.61％-103.25％,and relative standard deviations(RSDs)of 1.65％-12.57％.The organic acid contents in 10 Shine Muscat grape samples were subsequently analyzed using our established method,and the resultant data were quite consistent with literature-reported ranges.As a pioneering application of HILIC columns for organic acid analysis in grapes,our highly sensitive simultaneous detection of the three target acids will provide robust technical support for high-throughput detection of hydrophilic organic acids.关键词
有机酸/HILIC色谱柱/拖尾因子/超高效液相色谱-串联质谱(UPLC-MS/MS)/葡萄Key words
organic acids/HILIC column/tailing factor/UPLC-MS/MS/grape分类
轻工纺织引用本文复制引用
吴琴燕,赵来成,李冬冬,梁红芳,郑翠,刘吉祥,王建华,陈露,饶斌,秦岭..基于亲水相互作用色谱柱的超高效液相色谱串联质谱法同时检测葡萄中3种有机酸[J].生物加工过程,2025,23(5):568-574,7.基金项目
镇江市科技计划(SH2024111) （SH2024111）
2025年度镇江市重大农业技术推广项目 （）
