山西医科大学学报2025，Vol.56Issue(9)：1006-1015,10.DOI:10.13753/j.issn.1007-6611.2025.09.004
番石榴叶提取物通过抑制VEGF信号通路减轻糖尿病小鼠胰岛细胞损伤
Guava leaf extract alleviates islet cell injury in diabetic mice by inhibiting VEGF signaling pathway
摘要
Abstract
Objective To investigate the effects of Psidium guajava leaf extract on glucose metabolism and islet cell function in mice with type 2 diabetes mellitus(T2DM),and explore its potential mechanisms.Methods Twelve KK-Ay diabetic mice were randomly divided into T2DM group and Psidium guajava leaf extract group(1 638 mg/kg).After 8 weeks of intervention,glucose tolerance was assessed by oral glucose tolerance test(OGTT),and pancreatic tissue sections were examined using hematoxylin-eosin(HE)staining to evaluate islet histology.Network pharmacology and bioinformatics analyses were conducted to identify shared genes and signaling pathways between the active ingredients of the extract and T2DM.Key protein expressions were validated by immunohistochemical staining.Results Compared with T2DM group,mice in Psidium guajava leaf extract group showed significantly reduced fasting blood glucose and decreased blood glucose levels at all time points during OGTT(P＜0.000 1).HE staining revealed obvious improvement in islet architecture.Quantitative immunohistochemistry showed significantly increased insulin expression in islets(P＜0.000 1).Network pharma-cology analysis identified 128 potential targets of the active compounds of the extract,of which 85 overlapped with T2DM-related proteins,mainly enriched in the vascular endothelial growth factor(VEGF)signaling pathway,and other metabolic regulatory pathways such as the thyroid hormone pathway.Immunohistochemical staining further demonstrated significantly reduced expressions of VEGFA,p-P38,and AKT in Psidium guajava leaf extract group compared with T2DM group(P＜0.01).Conclusion Psidium guajava leaf extract may alleviate islet cell injury and improve glucose metabolism in diabetic mice by inhibiting the VEGF signaling pathway.关键词
2型糖尿病/番石榴叶提取物/网络药理学/生物信息学/VEGF信号通路/胰岛功能/葡萄糖代谢Key words
type 2 diabetes mellitus/Psidium guajava leaf extract/network pharmacology/bioinformatics/VEGF signaling pathway/islet function/glucose metabolism分类
医药卫生引用本文复制引用
邓玲,阎飞帆,冯昌梅,杨琼..番石榴叶提取物通过抑制VEGF信号通路减轻糖尿病小鼠胰岛细胞损伤[J].山西医科大学学报,2025,56(9):1006-1015,10.基金项目
国家自然科学基金项目(82060671) （82060671）
广西自然科学基金项目(2020GXNSFBA238001) （2020GXNSFBA238001）
