现代检验医学杂志2025，Vol.40Issue(6)：110-115,6.DOI:10.3969/j.issn.1671-7414.2025.06.020
NSCLC患者外周血自然细胞毒性受体NKp46,NKp30,NKG2A和NKG2D表达水平及临床价值研究
Expression Levels of NKp46,NKp30,NKG2A and NKG2D on Natural Cytotoxicity Receptor in Peripheral Blood of NSCLC Patients and Its Clinical Diagnostic Value
摘要
Abstract
Objective To analyze the expression levels of natural cytotoxicity receptor(NCR)NKp46,NKp30,NKG2A and NKG2D in peripheral blood of non-small cell lung cancer(NSCLC)patients and its clinical diagnostic value.Methods A total of 50 NSCLC patients admitted to Affiliated Shijiazhuang Ping'an Hospital of Hebei University of Chinese Medicine from October 2021 to August 2022 were selected as the study group(NSCLC group).Meanwhile,20 healthy individuals who underwent physical examinations during the same period were selected as the control group.Peripheral blood samples were collected and the positive expression rate of NKp4,NKp30,NKG2A and NKG2D on NK cell surface was detected by flow cytometry.Clinical data from patients was collected and the correlation between the positive expression rate of NKp46,NKp30,NKG2A and NKG2D and pathological type,TNM staging and survival time were analyzed by One-way ANOVA/t test.Results Compared to control group,the positive expression rate of NKp46 on NK cells in peripheral blood of NSCLC patients were significantly elevated(42.09%±18.55%vs 25.92%±14.03%),the difference was statistically significant(t=3.511,P＜0.01).Comparison of the positive expression rates of NKG2A,NKG2D and NKp30 between the two groups and the differences were not statisticany significant(t=0.447,0.536,1.941,all P＞0.05).Subgroup analysis of NK cells revealed a significant increase in the proportion of CD56brightCD16+NK subgroups in NSCLC group compared to control group(3.76%±1.91%vs 2.42%±0.85%),the difference was statistically significant(t=3.017,P＜0.01).The positive expression rate of NKp46 in the CD56brightCD16+NK subgroup and CD56dimCD16+NK subgroup significantly increased(59.64%±21.12%vs 43.91%±16.04%,51.32%±19.84%vs 38.69%±15.12%),the differences were statistically significant(t=2.999,2.562,all P＜0.01).There was no significant difference in the positive expression rate of NKp46,NKG2A,NKG2D and NKp30 among squamous cell carcinoma and adenocarcinoma in lung(t=0.188～0.600,all P＞0.05).As TNM progresses,the positive expression rate of NKp46 on peripheral blood NK cells in NSCLC patients decreases,but the trend is of no statistical difference(F=2.381,P=0.10).Compared to patients with survival period≤12 months,the positive expression rate of NKp46 in patients with survival period>12 months was significantly higher(49.77%±17.52%vs 36.71%±15.41%),the difference was statistically significant(t=2.716,P＜0.01).Conclusion The positive expression rate of NKp46 in peripheral blood NK cells of NSCLC patients is increased,mainly in the CD56dimCD16+NK subgroup and CD56brightCD16+NK subgroup.The expression of NKp46 is related to the survival time of NSCLC patients,but not to the pathological type.Peripheral blood NCR NKp46 can serve as a potential biomarker for the diagnosis and prognostic evaluation of NSCLC.关键词
非小细胞肺癌/自然杀伤细胞/自然细胞毒性受体/流式细胞术Key words
non-small cell lung cancer/natural killer cell/natural cytotoxicity receptor/flow cytometry分类
临床医学引用本文复制引用
李雅楠,杨会涛,谷贺,李进..NSCLC患者外周血自然细胞毒性受体NKp46,NKp30,NKG2A和NKG2D表达水平及临床价值研究[J].现代检验医学杂志,2025,40(6):110-115,6.基金项目
石家庄市科学技术研究与发展计划项目(211200763). （211200763）
