现代检验医学杂志2025，Vol.40Issue(6)：196-200,5.DOI:10.3969/j.issn.1671-7414.2025.06.036
间接法建立深圳地区育龄期健康女性血清抗缪勒氏管激素参考区间及验证
Application of Indirect Method in the Establishment Reference Interval for Anti-Miillerian Hormone in Healthy Women of Childbearing Age in Shenzhen Area
摘要
Abstract
Objective To establish and validate the reference interval for serum anti-Müllerian hormone(AMH)in healthy women of childbearing age in Shenzhen using an indirect method based on mathematical statistics.Methods Collected the AMH data for women aged 21～50 in outpatient and physical examination populations in Shenzhen Maternity and Child Health Care Hospital from 2017 to 2023.Grouping by age range of five,first performed normality tested on each group of data,and used the interquartile range method to remove outliers for non-normal data.Then established reference intervals for AMH in different age groups through indirect method(Hoffmann method)and verified them.Compared with the reference interval of the reagent instructions and analyzed the correlation be-tween serum AMH levels and age.Results The correlation coefficient between serum AMH and age in women of childbearing age was-0.642,and the difference was statistically significant(P<0.001).The distribution of AMH levels among women in six age groups was compared,and the difference was statistically significant(H=28 392.655,P<0.05),and AMH levels showed a decreasing trend with age.The reference range of serum AMH at ages 21～25,26～30,31～35,36～40,41～45 and 46～50 yesrs were 0.92～11.30,0.68～9.43,0.38～7.51,0.12～6.93,<4.42 and<1.83ng/ml,respectively,and all reference intervals in each group had been validated.Compared to the age groups provided by the manufacturer,the age groups in this study are more refined,the reference range was narrower,and the interpretation of clinical and laboratory data was more accurate.Conclusion This study used Hoffmann's indirect method for the first time to establish a reference range for AMH in women aged 21～50 years in Shenzhen.The reference interval established is more in line with the actual situation and is a simple and reliable acquisition mode,suitable for promotion and wide application in clinical labo-ratories.关键词
Hoffmann法/间接法/抗缪勒氏管激素/参考区间/深圳地区Key words
Hoffmann method/indirect method/anti-Miillerian hormone/reference intervals/Shenzhen area分类
医药卫生引用本文复制引用
秦晓林,段纯,李俊祥,高宇驰,杨婕,谭健辉,马庆林..间接法建立深圳地区育龄期健康女性血清抗缪勒氏管激素参考区间及验证[J].现代检验医学杂志,2025,40(6):196-200,5.基金项目
深圳市科技计划项目(JCYJ20220530155213031) （JCYJ20220530155213031）
广东省基础与应用基础研究基金项目(2020A1515010590). （2020A1515010590）
