时珍国医国药2025，Vol.36Issue(21)：4010-4016,7.DOI:10.70976/j.1008-0805.SZGYGY-2025-2102
肾衰营养胶囊调节炎症及肌分化改善慢性肾脏病大鼠骨骼肌萎缩的机制研究
Shenshuai Yingyang Capsule(肾衰营养胶囊)regulates inflammation and skeletal muscle dif-ferentiation to ameliorate skeletal muscle atrophy in chronic kidney disease rats
摘要
Abstract
Objective To investigate the mechanism by which Shenshuai Yingyang Capsule(肾衰营养胶囊,SSYYC)alleviates skel-etal muscle atrophy in rats with chronic kidney disease(CKD).Methods The CKD rat model was established by 5/6 nephrectomy.Suc-cessfully modeled rats were randomly divided into a model group and a SSNC group,with 8 rats in each group;a group(n=8)was estab-lished.After 10 weeks of treatment,serum creatinine(Scr),urea nitrogen(BUN),serum albumin(Alb),and inflammatory marker levels(IL-1β,IL-6,CRP,TNF-α)were measured in each group.The skeletal muscle morphology of rats in each group was observed using HE staining.Target genes were identified using transcriptome sequencing,and differentiation protein levels were detected by Western blot(WB).Results Compared with the sham surgery group,after 8 weeks,the model group showed significantly decreased renal function,upregulated serum inflammatory indicators(IL-1β,IL-6,CRP,TNF-α),reduced cross-sectional area of skeletal muscle fibers,and downregulated MyoD and Myf4 protein levels.Compared with the model group,the SSYYC intervention group showed improved renal function and decreased levels of inflammatory factors;the cross-sectional area of skeletal muscle fibers increased(P<0.05),and MyoD and Myf4 protein levels were upregulated(P<0.05).Transcriptome sequencing results showed 201 differen-tially expressed genes(DEGs)in skeletal muscle tissue between the model group and the SSYYC group.Among these,44 were target genes,including Fbxo32 and Tmco3.Gene enrichment analysis suggested an association with pathways regulating skeletal muscle myo-genesis,differentiation,and metabolism.Conclusion SSYYC alleviates skeletal muscle inflammation and atrophy in CKD rats,which may be related to the regulation of MyoD and Myf4.关键词
慢性肾脏病/骨骼肌萎缩/肾衰营养胶囊/炎症与骨骼肌分化/转录组测序Key words
Chronic kidney disease/Skeletal muscle atrophy/Shenshuai Yingyang Capsule(肾衰营养胶囊)/Inflammation and skeletal muscle differentiation/Transcriptome sequencing分类
医药卫生引用本文复制引用
赵树茗,王明清,魏连波,胡蓉..肾衰营养胶囊调节炎症及肌分化改善慢性肾脏病大鼠骨骼肌萎缩的机制研究[J].时珍国医国药,2025,36(21):4010-4016,7.基金项目
国家自然科学基金(82104795) （82104795）
广东省中医药局科研项目(20221255) （20221255）
广州市科技计划项目(202201011438) （202201011438）
广东省自然科学金(2025A1515010577) （2025A1515010577）
