时珍国医国药2025，Vol.36Issue(21)：4033-4038,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-2105
四君子汤及其不同组分脾虚小鼠含药血清对IEC-6和PC12细胞的影响
Effects of Sijunzi Decoction(四君子汤)and its different component medicated serums from spleen-deficiency mice on IEC-6 and PC12 cells
摘要
Abstract
Objective To compare effects of Sijunzi Decoction(四君子汤,SD)and its different components based on the medicated serum of spleen deficiency mice and cell models.Methods An IEC-6 cell scratch model and a corticosterone-induced PC12 cell injury model were established.Blank and model group cells were treated with blank or spleen-deficient mouse serum,while the treatment groups were administered drug-containing serum from spleen-deficiency mice treated with SD,non-polysaccharide fraction of SD(NPS),or active fraction of SD(ECS).The migration of IEC-6 cells was recorded,and Western blot(WB)was used to detect the pro-tein expression levels of the PI3K/AKT signaling pathway in IEC-6 cells.PC12 cell viability was measured using the Cell Counting Kit-8(CCK-8)assay,and lactate dehydrogenase(LDH)release rate was determined by ELISA.Results Compared with the model group of IEC-6 cells,IEC-6 cells in SD group,NPS group and ECS group showed significant migration phenomenon(P<0.05),and NPS medi-cated serum showed better effect than SD and ECS on promoting the migration.All three medicated serum could activate PI3K/AKT sig-naling pathway of IEC-6 cells(P<0.05)to repairing the intestinal epithelial cell injury.Compared with the model group of PC12 cells,SD,NPS and ECS medicated serum significantly improved corticosterone-induced PC12 cell injury(P<0.05)and reduced LDH release rate of PC12 cells(P<0.05).Conclusion Drug-containing serum from spleen-deficiency mice treated with SD and its different compo-nents exerts protective effects on IEC-6 and PC12 cell injury.The efficacy of ECS is comparable to that of SD,suggesting it may be the active fraction.The PI3K/AKT pathway may be one of the mechanisms by which ECS repairs intestinal epithelial cells.关键词
四君子汤/脾虚证/血清药理/IEC-6/PC12Key words
Sijunzi Decoction(四君子汤)/Spleen deficiency syndrome/Serum pharmacology/IEC-6/PC12分类
医药卫生引用本文复制引用
沈怡,彭颖,陈孝男,董邦健,李晓波..四君子汤及其不同组分脾虚小鼠含药血清对IEC-6和PC12细胞的影响[J].时珍国医国药,2025,36(21):4033-4038,6.基金项目
国家自然科学基金(81973441) （81973441）
