时珍国医国药2025，Vol.36Issue(21)：4047-4052,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-2107
温阳通便汤调控PINK1/Parkin通路治疗功能性便秘的机制研究
Mechanism of Wenyang Tongbian Decoction(温阳通便汤)in alleviating functional constipa-tion via regulating the PINK1/Parkin pathway
摘要
Abstract
Objective To investigate the mechanism by which Wenyang Tongbian Decoction(温阳通便汤,WYTBD)alleviates func-tional constipation(FC)through regulating the PINK1/Parkin signaling pathway to inhibit apoptosis.Methods C57BL/6 mice were ran-domly divided into the control group,model group,low/medium/high-dose WYTBD groups,and the lactulose group.Except for the con-trol group,all other groups were administered loperamide hydrochloride by gavage once daily for one week to establish the FC model.Following successful model establishment,the mice were treated with graded doses of WYTBD or lactulose for one week.Subsequently,the number of fecal pellets,fecal water content,and intestinal transit rate were measured.HE staining was used to observe pathological changes in colon tissue,TUNEL staining was used to detect colonocyte apoptosis,and Western blot(WB)was performed to measure the expression of PINK1,Parkin,Bcl-2,Bax,and Caspase-3 proteins in colon tissue.Results WYTBD significantly increased the number of fecal pellets,fecal water content,and intestinal transit rate in constipated mice(P<0.05 or P<0.01),ameliorated pathological changes in colon tissue,inhibited colonocyte apoptosis,upregulated the expression of PINK1,Parkin,and Bcl-2 proteins(P<0.05),and downregulated the expression of Bax and Caspase-3 proteins(P<0.05).Conclusion WYTBD alleviates FC by regulating the PINK1/Parkin pathway to inhibit apoptosis.关键词
温阳通便汤/功能性便秘/PINK1/Parkin通路/细胞凋亡Key words
Wenyang Tongbian Decoction(温阳通便汤)/Functional constipation/PINK1/Parkin pathway/Apoptosis分类
医药卫生引用本文复制引用
张晓雨,颜克秋,孙光军,刘洪涛,肖文洁,周金波,蔡易凡,林爱珍..温阳通便汤调控PINK1/Parkin通路治疗功能性便秘的机制研究[J].时珍国医国药,2025,36(21):4047-4052,6.基金项目
湖北省科技厅重点研发专项(2023BCB031) （2023BCB031）
湖北中医药大学博士研究生创新能力提升计划项目(2025BSCX12) （2025BSCX12）
武汉市肛肠疾病中西医结合治疗临床医学研究中心项目(武科[2023]123号) （武科[2023]123号）
