时珍国医国药2025，Vol.36Issue(21)：4053-4058,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-2108
姜黄素调节HIF-1α/VEGFR/Akt通路对肺癌细胞增殖、凋亡和血管生成的影响
Effects of curcumin on proliferation,apoptosis,and angiogenesis of lung cancer cells via regu-lating the HIF-1α/VEGFR/Akt pathway
摘要
Abstract
Objective To investigate the effects of curcumin on proliferation,apoptosis,angiogenesis,and the hypoxia-inducible factor-1α/vascular endothelial growth factor receptor/protein kinase B(HIF-1α/VEGFR/Akt)pathway in lung cancer cells.Methods A549 lung cancer cells were cultured and divided into the following groups:the control group(Control),curcumin low/medium/high-concentration treatment groups(CUR-L/M/H),and a curcumin high-concentration treatment combined with pathway activator dimethyl-oxalylglycine group(CUR-H+DMOG).Cell proliferation was detected using the Edu assay;cell apoptosis was measured by flow cytom-etry(FCM);angiogenesis was observed;and the expression of vascular endothelial growth factor A(VEGFA)and proteins related to the HIF-1α/VEGFR/Akt pathway was determined by Western blot(WB).Results Compared with the Control group,the CUR-L/M/H groups showed reduced angiogenic lumen formation,decreased Edu-positive rates,and lower expression levels of CyclinD1,Bcl-2,VEGFA,HIF-1α,VEGFR2,and p-AKT/AKT,while the apoptosis rate and expression of Bax and p21 were increased(P<0.05).Compared with the CUR-H group,the CUR-H+DMOG group exhibited increased angiogenic lumen formation,elevated Edu-positive rates,and higher expression of CyclinD1,Bcl-2,VEGFA,HIF-1α,VEGFR2,and p-AKT/AKT,whereas the apoptosis rate and expression of Bax and p21 were decreased(P<0.05).Conclusion Curcumin may inhibit proliferation and angiogenesis,and promote apoptosis in lung cancer cells,mechanisms that could be associated with the suppression of the HIF-1α/VEGFR/Akt pathway.关键词
姜黄素/缺氧诱导因子-1α/血管内皮细胞生长因子受体/蛋白激酶通路/肺癌/增殖/凋亡/血管生成Key words
Curcumin/Hypoxia-inducible factor-1α/vascular endothelial growth factor receptor/protein kinase B pathway/Lung cancer/Proliferation/Apoptosis/Angiogenesis分类
医药卫生引用本文复制引用
焦会茹,李丹青,谢瑾,杨洪喜..姜黄素调节HIF-1α/VEGFR/Akt通路对肺癌细胞增殖、凋亡和血管生成的影响[J].时珍国医国药,2025,36(21):4053-4058,6.基金项目
河北省医学科学研究课题计划(20232020) （20232020）
