时珍国医国药2025，Vol.36Issue(21)：4059-4065,7.DOI:10.70976/j.1008-0805.SZGYGY-2025-2109
宫粘方通过提高子宫内膜容受性治疗宫腔粘连的机制研究
Study on the mechanism of Gongzhan Formula(宫粘方)in treating intrauterine adhesions by enhancing endometrial receptivity
摘要
Abstract
Objective To investigate the mechanism of Gongzhan Formula(宫粘方,GZF)in the treatment of intrauterine adhesions(IUA).Methods In vivo:Forty-five SD rats were randomly assigned to the sham surgery group(SHAM),amodel group(MOD),or the GZF group.Pathological changes in rat endometrial tissues were assessed by HE staining on postoperative days 1,3,5,10 and 28.The expression of integrin αvβ3 was evaluated by immunohistochemistry(IHC).In vitro:Endometrial stromal cells(ESCs)and macro-phages(M)were isolated and cultured from the endometrium of IUA model rats(n=3 per cell type).Cells cultured with serum contain-ing GZF were designated as the experimental groups(M+HYXQ and ESC+HYXQ).IF was used to detect the expression of cytokeratin and vimentin.CCK-8 assay was employed to assess ESC proliferation.Angiogenesis was evaluated using a tube formation assay.The mRNA expression of CD51 was measured by qRT-PCR.Results HE staining revealed significantly reduced gland number,increased inflammatory cell infiltration,and sparse and incomplete tissue structure in the MOD group endometrium compared to the SHAM group(P<0.05).In contrast,the GZF group showed alleviated inflammatory reaction,more complete and dense endometrial structure,and increased gland number(P<0.05).IHC demonstrated significantly higher expression of integrin αvβ3 in the GZF group compared to the MOD group(P<0.05).IF indicated significantly higher vimentin expression compared to cytokeratin in the endometrium of IUA model rats.In the ESC+HYXQ and M+HYXQ groups,stromal cell absorbance(indicating viability),proliferation rate,and the number of vascular lumens and nodes were significantly increased.Furthermore,CD51 mRNA expression was also significantly elevated in these groups(P<0.05).Conclusion GZF may promote gland formation,alleviate endometrial inflammation,and upregulate integrin αvβ3 expression,thereby enhancing endometrial stromal cell proliferation and angiogenesis.These effects potentially contribute to improved endometrial receptivity and the treatment of IUA.关键词
宫腔粘连/宫粘方/子宫内膜容受性/αvβ3Key words
Intrauterine adhesions/Gongzhan Formula(宫粘方)/Endometrial receptivity/Integrin αvβ3分类
医药卫生引用本文复制引用
王莹,金晶,邓阿黎,熊梦欣,姜惠中,向英,吴双,陈颖庆..宫粘方通过提高子宫内膜容受性治疗宫腔粘连的机制研究[J].时珍国医国药,2025,36(21):4059-4065,7.基金项目
全国名中医传承工作室建设项目(国中医药办人教函[2022]245号) （国中医药办人教函[2022]245号）
湖北省自然科学基金(2022CFD154 （）
2023AFD132 （）
2023AFD180) （）
国家自然科学基金青年基金(82305350) （82305350）
