生殖医学杂志2025，Vol.34Issue(10)：1350-1358,9.DOI:10.3969/j.issn.1004-3845.2025.10.008
血清IL-6/JAK2/STAT3信号通路水平与早发性卵巢功能不全患者卵巢反应性及IVF-ET妊娠结局的关系研究
Relationships among serum IL-6/JAK2/STAT3 signaling pathway levels and ovarian responsiveness and pregnancy outcomes of IVF-ET in patients with premature ovarian insufficiency
摘要
Abstract
Objectives:To investigate the relationships among serum interleukin-6(IL-6)/Janus kinase-2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway levels and ovarian response and pregnancy outcomes of in vitro fertilization-embryo transfer(IVF-ET)in patients with premature ovarian insufficiency(POI). Methods:A total of 196 POI patients who underwent IVF-ET at Xingtai Central Hospital from January 2021 to August 2024 were selected as the POI group,and 98 healthy women who underwent physical examinations during the same period were selected as the control group in a 2∶1 ratio.Serum IL-6,JAK2 and STAT3 levels in two groups were measured by enzyme-linked immunosorbent assay.POI patients were further divided into high response group(26 cases),normal response group(58 cases)and low response group(112 cases)based on ovarian responsiveness during assisted reproduction.According to the pregnancy outcomes of IVF-ET,the patients were divided into adverse outcome group(112 cases)and good outcome group(84 cases).Correlation analyses of serum IL-6,JAK2 and STAT3 levels in POI patients were conducted using Pearson correlation coefficient.The relationship between serum IL-6/JAK2/STAT3 signaling pathway levels and IVF-ET pregnancy outcomes in POI patients were analyzed by multivariate logistic regression.The predictive efficiency of the serum IL-6/JAK2/STAT3 signaling pathway levels for poor IVF-ET pregnancy outcomes in POI patients was analyzed by receiver operating characteristic(ROC)curve. Results:Compared with the control group,serum levels of IL-6,JAK2 and STAT3 were significantly increased in the POI group(P＜0.001).The serum levels of IL-6,JAK2 and STAT3 of POI patients showed pairwise correlations(P＜0.001).The serum levels of IL-6,JAK2 and STAT3 were all gradually increased from the low to high response group(P＜0.001).The poor IVF-ET pregnancy outcome rate in 196 POI patients was 57.14％(112/196).The elevated FSH level,abnormal ovarian hyporesponsiveness,the increase of IL-6 level,JAK2 level and STAT3 level were independent risk factors for poor IVF-ET pregnancy outcomes in POI patients,while the elevated anti-Müllerian hormone(AMH)was an independent protective factor(P＜0.05).The area under the ROC curve for predicting poor IVF-ET pregnancy outcomes using the combined IL-6/JAK2/STAT3 signaling pathway in POI patients was 0.931,which was higher than that predicted by serum IL-6,JAK2,STAT3 levels alone(0.771,0.785,0.790,respectively,P＜0.001),with the sensitivity of 0.866 and the specificity of 0.857. Conclusions:The increase of serum IL-6/JAK2/STAT3 signaling pathway levels were associated with decreased ovarian response and poor IVF-ET pregnancy outcomes in POI patients.The combination of serum IL-6/JAK2/STAT3 signaling pathway levels showed a high predictive power for adverse pregnancy outcomes in IVF-ET.关键词
早发性卵巢功能不全/IL-6/JAK2/STAT3信号通路/卵巢反应性/IVF-ET/妊娠结局Key words
Premature ovarian insufficiency/IL-6/JAK2/STAT3 signaling pathway/Ovarian response/In vitro fertilization-embryo transfer/Pregnancy outcomes分类
临床医学引用本文复制引用
霍森烨,霍艺超,王新月,樊秀梅,高妍,乔艳华,成云云,高敏..血清IL-6/JAK2/STAT3信号通路水平与早发性卵巢功能不全患者卵巢反应性及IVF-ET妊娠结局的关系研究[J].生殖医学杂志,2025,34(10):1350-1358,9.基金项目
河北省卫生健康委科研基金项目(2020-0444) （2020-0444）
邢台市重点研发计划自筹项目(2020ZC346) （2020ZC346）
