土壤学报2025，Vol.62Issue(5)：1523-1534,12.DOI:10.11766/trxb2024100150
长期秸秆还田对土壤丛枝菌根真菌及其生态网络的影响
Effects of Long-term Straw Return on Soil Arbuscular Mycorrhizal Fungi and Their Ecological Network
摘要
Abstract
[Objective]Arbuscular mycorrhizal fungi(AMF)play a crucial role in soil microbial ecological networks and can form symbiotic structures with about 80%of plants,making them a key component of sustainable soil management.[Methods]In order to reveal the response mechanism of soil AMF and their ecological network to straw return in the fluvo-aquic soil area of North China Plain,experimental plots with different straw return treatments were chosen under long-term wheat-maize rotation,and high-throughput sequencing and ecological network analyses were applied to elucidate the composition of soil mycorrhizal practices and fertilization treatments.The experiment was set up with five treatments and three replications,including T1:straw removal+PK fertilizer;T2:straw mulching+PK fertilizer;T3:straw removal+NPK fertilizer;T4:straw mulching+NPK fertilizer;and T5:straw burying+NPK fertilizer.[Results]The results showed that:(1)Straw incorporation with NPK fertilizer significantly decreased soil pH and increased the content of soil organic matter,total nitrogen,ammonium nitrogen,available potassium,and available phosphorus(P<0.05).This suggests that the combination of straw and NPK fertilizer is effective in enhancing soil fertility.(2)Different treatments of straw burial and nitrogen fertilizer application significantly affected the community composition of AMF,however,there was no significant difference in the alpha diversity of the communities.Nevertheless,Glomus and Paraglomus were t he main dominant genera of t he AMF community i n t he fluvo-aquic soil,highlighting their important role in these ecosystems.(3)Co-occurrence network analysis revealed seven distinct ecological network modules.Notably,the species abundance in module Ⅵ showed a significant positive correlation with several soil nutrient indices,including organic matter,nitrate nitrogen,available phosphorus,available potassium,and total nitrogen(P<0.05).This module also exhibited a significant positive correlation with N-acetylglucosaminidase activity,an enzyme involved in nitrogen cycling(P<0.05).Conversely,the species abundance in module Ⅵ was significantly negatively correlated with soil pH(P<0.05).[C onclusion]Different straw returning methods can effectively regulate soil fertility by improving soil physicochemical properties,modulating the structure and diversity of soil microbial communities,and adjusting AMF network interactions.By understanding the complex interactions between AMF communities and soil management practices,we can develop more effective strategies for maintaining soil health and productivity.This research provides valuable insights into the mechanisms by which straw returning and fertilization t reatments i nfluence AMF communities and t heir ecological networks,offering a foundation for future studies and practical applications in sustainable agriculture.关键词
秸秆还田/丛枝菌根真菌/土壤肥力/网络分析Key words
Straw return/Arbuscular mycorrhizal fungi/Soil fertility/Network analysis分类
农业科技引用本文复制引用
施启欢,张艳杰,周桂香,张佳宝,张丛志,鲁顺保,贾春花,刘明凤..长期秸秆还田对土壤丛枝菌根真菌及其生态网络的影响[J].土壤学报,2025,62(5):1523-1534,12.基金项目
国家重点研发计划项目(2022YFD1500401、2022YFD1500203)和现代农业产业技术体系专项资金(CARS-03-15、CARS-52)资助 Supported by the National Key R&D Program of China(Nos.2022YFD1500401,2022YFD1500203)and the China Agriculture Research System(Nos.CARS-03-15,CARS-52) （2022YFD1500401、2022YFD1500203）
