土壤学报2025，Vol.62Issue(5)：1571-1584,14.DOI:10.11766/trxb202410220409
覆盖种植下土壤中代谢物-微生物及枸杞产量的响应
Response of Soil Metabolites,Microorganisms,and Lycium barbarum Yield Under Cover Crop Cultivation
摘要
Abstract
[Objective]The global wolfberry(Lycium barbarum L.)industry is undergoing a paradigm shift from traditional Ningxia-dominated production to multipolar cultivation systems across Northwest China.In this transitional context,developing sustainable soil management strategies becomes imperative for maintaining agricultural productivity and ecosystem resilience.While cover crop intercropping has emerged as an advanced agroecological practice demonstrating dual benefits in fruit quality enhancement and environmental stewardship,conventional field management approaches;particularly long-term monoculture and chemical fertilizer overapplication,continue to compromise both the yield and phytochemical quality of wolfberry.This study systematically investigates the rhizosphere engineering effects of wolfberry/forage radish(Raphanus sativus L.)intercropping coupled with organic fertilization,focusing on its mechanistic impacts on soil microbiome restructuring and metabolic reprogramming.[Method]This study investigated the effects of wolfberry(Lycium barbarum L.)/radish(Raphanus sativus L.)intercropping with manure on edaphic microbial communities and metabolite profiles through a split-plot field experiment(2019-2021)in arid northwestern China.It also considered the effects of cover crop planting patterns on soil microbial community structure,metabolite composition,and yield of wolfberry orchards.Three organic fertilization regimes(0,6 660 kghm-2,and 13 320 kghm-2)were applied under two planting systems:monoculture(M)and intercropping(I).Using Illumina high-throughput sequencing technology,quantitative PCR methods,and liquid chromatography-tandem mass spectrometry(LC-MS),microbial information and metabolites were measured.The correlation between soil physicochemical properties,microbial diversity,key metabolite components,crop yield and its differential metabolites,microbial diversity index,and environmental factors under different planting modes was analyzed.[Result]Compared with the traditional wolfberry monoculture,intercropping significantly enhanced soil multifunctionality,increasing surface soil electrical conductivity(EC)by 29.66%,organic carbon(SOC)by 47.80%,total nitrogen(TN)by 39.09%,available nitrogen(AN)by 46.23%,available potassium(AK)by 36.64%,and microbial biomass nitrogen(MBN)by 22.56%compared with monoculture(P<0.05).Additionally,intercropping enhanced soil microbial diversity,with the bacterial Shannon index increasing by 4%and the Simpson index decreasing by 43.04%.Metabolomic analysis identified 867 metabolites,including 84 differentially abundant compounds such as lipids,organic acids,phenylpropanoids,and carbohydrates.Among these,two key metabolites were positively correlated with yield enhancement.Consequently,wolfberry yield increased significantly by 6.36%.[Conclusion]These findings indicated that cover crop intercropping improved soil microecological conditions,induced specific metabolite shifts,and effectively enhanced wolfberry productivity,demonstrating its applicability in sustainable wolfberry cultivation systems.关键词
枸杞/肥田萝卜/覆盖作物/有机肥/微生物多样性/代谢组Key words
Lycium barbarum/Forage radish/Cover crops/Organic fertilizer/Microbial diversity/Metabolome分类
农业科学引用本文复制引用
陈浩楠,马丫然,高亚苗,南雄雄,朱丽珍,杨柳,王芳..覆盖种植下土壤中代谢物-微生物及枸杞产量的响应[J].土壤学报,2025,62(5):1571-1584,14.基金项目
宁夏自然科学基金项目(2024AAC03129,2024AAC03768)和国家自然科学基金项目(42067022)资助 Supported by the Natural Science Foundation of Ningxia,China(Nos.2024AAC03129 and 2024AAC03768),and the National Natural Science Foundation of China(No.42067022) （2024AAC03129,2024AAC03768）
