畜牧兽医学报2025，Vol.56Issue(10)：4796-4806,11.DOI:10.11843/j.issn.0366-6964.2025.10.004
取样后动物胚胎的冷冻保存技术研究进展
Research Progress on Cryopreservation Technology of Animal Embryos after Sampling
摘要
Abstract
Post-sampling embryo cryopreservation technology is a critical component of preim-plantation genetic testing(PGT),holding significant application value in embryo sex selection,genetic screening,genomic selection,and assisted reproduction.However,embryonic sampling procedures may lead to zona pellucida damage,reduced cell count,and epigenetic abnormalities,thereby limiting cryopreservation efficiency.Recent advancements,including optimized sampling methods(e.g.,polar body biopsy,blastomere biopsy,and trophectoderm biopsy),improved culture medium formulations(e.g.,supplementation with L-arginine and glutathione),and re-fined cryopreservation protocols(e.g.,vitrification techniques),have markedly enhanced the cryopreservation efficiency of post-sampling embryos.Furthermore,non-invasive techniques(e.g.,analysis of cell-free DNA in embryo culture medium)and epigenetic modifications(e.g.,DNA methylation regulation)offer novel approaches to minimize embryonic damage.This review summarizes the mechanisms of cryopreservation-induced damage in post-sampling embryos and protective strategies,with a focus on cutting-edge advancements in zona pellucida repair,epige-netic regulation,and cryopreservation efficiency.By evaluating the strengths and limitations of existing technologies,this review aims to provide theoretical foundations and technical references for developing safe and efficient cryopreservation protocols,thereby promoting their application and adoption in livestock breeding and assisted reproductive technologies.关键词
胚胎取样/PGT/冷冻保存/冷冻效率Key words
embryo sampling/PGT/cryopreservation/freezing efficiency分类
农业科技引用本文复制引用
董建华,吕丽华,赵学明,杨柏高,张笑梦,冯肖艺,宋浩然,刘阳,王子卓,王彦博,李崇阳..取样后动物胚胎的冷冻保存技术研究进展[J].畜牧兽医学报,2025,56(10):4796-4806,11.基金项目
国家重点研发计划政府间重点专项(2022YFE0100200) （2022YFE0100200）
农业农村部和财政部资助:现代农业产业技术体系资助(CARS-36) （CARS-36）
国家自然科学基金国际合作项目(32161143032) （32161143032）
国家家养动物种质资源库 （）
中国农业科学院科技创新工程(ASTIP-IAS06) （ASTIP-IAS06）
