畜牧兽医学报2025，Vol.56Issue(10)：4925-4937,13.DOI:10.11843/j.issn.0366-6964.2025.10.014
基于转录组测序分析METTL14对绵羊骨骼肌卫星细胞成肌分化的影响
The Effect of METTL14 on Myogenic Differentiation of Ovine Skeletal Muscle Satellite Cells Based on Transcriptome Sequencing Analysis
摘要
Abstract
The aim of this study was to explore the molecular mechanism underlying the effect of methyltransferase like 14(METTL14)on the myogenic differentiation of sheep skeletal muscle satellite cells(SMSCs)and to provide a theoretical basis for analyzing the molecular mechanism of METTL14 regulating the differentiation of SMSCs.SMSCs were isolated and identified.The overexpression plasmid of METTL14 and the control plasmid were transfected into SMSCs,and the overexpression efficiency and the function of METTL14 affecting SMSCs differentiation were detected by RT-qPCR and Western blot.RNA-seq was performed for METTL 14-overexpressed SMSCs(OE-14)and control SMSCs(OE-NC).Differentially expressed genes(DEGs)were iden-tified under the conditions of P＜0.05 and log2|Fold Change|＞1,GO and KEGG enrichment analysis and protein-protein interaction(PPI)network interaction analysis for DEGs was conduc-ted.Overexpression of METTL14 markedly elevated the expression of METTL14,as well as the mRNA and protein levels of myogenic differentiation-related genes(MyHC,MyoG)in ovine SMSCs,thereby demonstrating successful METTL14 overexpression and the enhancement of SMSCs differentiation mediated by METTL14.A total of 259 differentially expressed genes were identified between the two groups.Compared with the OE-NC group,the OE-14 group had 45 up-regulated genes and 214 down-regulated genes.Differential genes were mainly enriched in nuclear pathways,Rap1 signaling pathways,etc.PPI protein network interaction analysis identi-fied 8 hub genes(Degree≥15),including MX 1,RSAD2,IFIH1,DDX58,HERC5,ISG15,MX2,and IRF7.This study indicated that METTL14 can promote the differentiation of ovine SMSCs through influencing genes and signaling pathways related to cellular processes and muscle development.关键词
METTL14/转录组测序/骨骼肌卫星细胞/绵羊/细胞分化Key words
METTL14/transcriptome sequencing/skeletal muscle satellite cells/sheep/cell dif-ferentiation分类
畜牧业引用本文复制引用
何思琦,陈倩,蒋琳,马月辉,周胜花,赵倩君..基于转录组测序分析METTL14对绵羊骨骼肌卫星细胞成肌分化的影响[J].畜牧兽医学报,2025,56(10):4925-4937,13.基金项目
国家自然科学基金面上项目(32172701) （32172701）
国家绒毛用羊产业技术体系项目(CARS-39-01) （CARS-39-01）
宁夏回族自治区自然科学基金(2023AAC02077) （2023AAC02077）
