畜牧兽医学报2025，Vol.56Issue(10)：4938-4946,9.DOI:10.11843/j.issn.0366-6964.2025.10.015
转录组分析揭示山羊角基皮肤的代谢适应性变化
Transcriptomic Analysis Reveals Metabolic Adaptive Changes in Goat Horned Skin
摘要
Abstract
This study aimed to reveal the adaptive changes in sex hormone secretion patterns and the metabolic mechanisms underlying the development of horn-supporting skin structures.Six adult buck,including 3 horned and 3 polled individuals,were selected.Blood samples were col-lected before morning feeding,and serum sex hormone levels were measured using enzyme-linked immunosorbent assay(ELISA)and radioimmunoassay(RIA).Skin samples were taken from the horn base of horned bucks and the corresponding forehead region of polled bucks for transcriptome sequencing analysis.Hormone analysis showed that the serum concentrations of progesterone,follicle-stimulating hormone(FSH),and luteinizing hormone(LH)were significantly lower in horned bucks than in polled bucks(P＜0.05).The testosterone-to-estradiol(T/E2)ratio was significantly higher in horned bucks(P＜0.05),but the levels of testosterone(T)and estradiol(E2)did not show significant differences.Transcriptome analysis identified 1 721 differ-entially expressed genes(DEGs),with 1 183 upregulated and 538 downregulated.These DEGs were significantly enriched in pathways related to metabolism(metabolic pathways),fatty acid elongation,fatty acid metabolism,extracellular matrix(ECM-receptor interaction),biosynthesis of unsaturated fatty acids,fatty acid degradation,and glycolipid metabolism.In conclusion,the findings suggest that horn-based skin requires more energy and keratin secretion for protein sheath growth,while maintaining higher structural stability by reducing cellular collagen and exoskeleton.In addition,horned bucks have reduced levels of P,FSH and LH,suggesting a shift in reproductive strategy towards horn-dependent morphological dominance and aggressive behaviour driven by high T/E2 ratios.The results of this study provide a theoretical basis for resolving the molecular regulatory mechanisms of horn growth and development in goats and the effects of horn traits on reproductive adaptations.关键词
山羊/无角性状/性激素/转录组Key words
goat/polled trait/sex hormones/transcriptome分类
畜牧业引用本文复制引用
黄岩,杨宇航,刘天伟,朱露,张思欢,凌英会..转录组分析揭示山羊角基皮肤的代谢适应性变化[J].畜牧兽医学报,2025,56(10):4938-4946,9.基金项目
财政部和农业农村部:国家肉羊产业技术体系(CAS38) （CAS38）
安徽省肉羊良种联合攻关(2021-2025) （2021-2025）
安徽省牛羊产业技术体系(AHN-YCYTX) （AHN-YCYTX）
