畜牧兽医学报2025，Vol.56Issue(10)：5095-5103,9.DOI:10.11843/j.issn.0366-6964.2025.10.029
鸡毒支原体LC株全基因组分析及中国流行株多位点序列分型分析
Whole-Genome Analysis of Mycoplasma gallisepticum LC Strain and Multilocus Sequence Typing of Prevalent Strains in China
摘要
Abstract
Mycoplasma gallisepticum(MG)infection causes chronic respiratory disease(CRD)in chickens,adversely affecting feed conversion efficiency,egg production rate,hatchability,and chick activity,leading to significant economic losses in the global poultry industry.This study aims to analyze the genomic characteristics and biological features of the LC strain and explore the epidemiological patterns of MG in China.Pipped failure embryos were collected in hatchery for MG isolation.The isolate underwent whole-genome sequencing(WGS)and analysis of adhesion-related protein variations.Additionally,multilocus sequence typing(MLST)analysis was con-ducted to determine the prevalence of MG strains in China.Genomic analysis revealed a compact MG genome of 953.4 kb with a GC content of 31.5％.Annotation identified 721 coding regions with an encoding density of 89.3％,33 tRNAs,and 2 CRISPR sequences.Functional enrichment in translation,metabolism,and signal transduction indicated efficient protein synthesis and envi-ronmental adaptability,with potential genes related to virulence regulation.Moreover,11 amino acid mutations were identified in the adhesion-related proteins P1,P30,and hwm3.MLST analy-sis classified the isolate as ST-81,along with ST-78 and ST-36,which constitute the prevalent MG genotypes circulating in China.MG has become as a significant contaminant in hatcheries,a-long with their genetic diversity notably increasing control challenges.This study provides in-sights into the genomic and biological characteristics of the LC strain and elucidates the dominant MG sequence types in China,offering valuable scientific evidence for pathogen biology research and vaccine strain selection.关键词
鸡毒支原体/啄壳不全活胚/基因组/氨基酸突变/多位点序列分型Key words
Mycoplasma gallisepticum/pipped failure embryo/genome/amino acid mutations/multi-locus sequence typing(MLST)分类
农业科技引用本文复制引用
方焕新,李琪,宋子昂,温家明,顾嘉运,王占新,覃健萍,于岩飞,张炜..鸡毒支原体LC株全基因组分析及中国流行株多位点序列分型分析[J].畜牧兽医学报,2025,56(10):5095-5103,9.基金项目
中央高校基本科研业务费专项资金资助(QTPY2025008) （QTPY2025008）
