畜牧兽医学报2025，Vol.56Issue(10)：5251-5265,15.DOI:10.11843/j.issn.0366-6964.2025.10.044
日照时数与奶牛骨关节疾病的相关性及光照紊乱对大鼠骨关节炎的影响
Correlation between Sunshine Duration and Osteoarthritis of Cows and the Effect of Light Disorder on Osteoarthritis in Rats
摘要
Abstract
This study aimed to explore the effects of light disturbance and biological clock on oste-oarthritis(OA)based on the relationship between the length of sunshine duration(SD)and oste-oarthritis(OA)in dairy farms in different regions.By investigating the incidence of bone and joint diseases of lactation cows and local monthly SD in 11 regions across the country from July 2018 to June 2021,the correlation between its incidence and SD was analyzed through Pearson.At the same time,64 SD male rats were randomly divided into N group(n=32)and OA group(n=32).The OA group used the method of anterior cruciate ligament cleavage(ACLT)to es-tablish an early OA model,and N group performed sham surgery.Each group is divided into two subgroups,and two light-dark period regulation modes(normal light LD and disordered light il-lumination Dis-LD)are established,namely N+LD,N+Dis-LD,OA+LD and OA+Dis-LD groups,with 16 individual groups.The test cycle was 6 weeks,and the weight changes in rats were recorded,the levels of inflammatory factors and cartilage degradation markers were detected in rat serum,and the articular cartilage was stained with frairo O-solid green and OARSI scores were used to detect the levels of proteins related to cartilage metabolism in rats,and the effects of key biological clock proteins in the heart,liver,spleen,lung and kidney of OA rats were detec-ted.Among the 11 regions surveyed,except for Yinchuan,the other 10 regions were positively correlated with SD,with 6 regions moderately positive correlation(0.4＜R2＜0.6)and 4 regions weakly correlated(0.2＜R2＜0.4).The results of rat experimental research showed that light disorders can significantly slow down the weight gain in rats,increase the content of proinflam-matory factors(IL-1β,TNF-α,IL-6,iNOS)and cartilage degradation markers(COMP and CTX-Ⅱ)in rats,promote the catabolism of articular cartilage matrix(MMP-3,MMP-13,AD-AMTS-4)in rats in OA group and reduce the synthesis of cartilage collagen fibers(COL2A1).The key biological clock proteins(BMAL1,NR1D1,CRY1 and PER3)in the heart,liver,spleen,lung and kidney of OA rats showed varying degrees.Epidemiological investigations re-vealed that the incidence of bone and joint diseases in dairy cows was significantly positively cor-related with SD.Rat experiments showed that light disorders lead to abnormal biological clocks,promote pathological damage to the cartilage and accelerate the development of OA.This experi-ment revealed the significant impact of light changes on bone and joint diseases in mammals,and provided a reference for dairy farms to develop appropriate artificial light cycle plans to reduce the incidence of bone and joint diseases in dairy cows in the future.关键词
光照紊乱/生物钟/骨关节疾病/日照时数/奶牛Key words
light disturbance/biological clock/bone joint disease/sunshine duration/dairy cattle分类
畜牧业引用本文复制引用
刘永醍,马天文,戚晶晶,郭晓艳,马玉辉,李淑欣,宋霄鹏,裘宗盛,高翔,李亚楠..日照时数与奶牛骨关节疾病的相关性及光照紊乱对大鼠骨关节炎的影响[J].畜牧兽医学报,2025,56(10):5251-5265,15.基金项目
国家自然科学基金青年项目(3240190616) （3240190616）
黑龙江省自然科学基金(LH2023C025) （LH2023C025）
