畜牧兽医学报2025，Vol.56Issue(10)：5328-5334,7.DOI:10.11843/j.issn.0366-6964.2025.10.050
2022-2024年中国部分地区牛病毒性腹泻病毒分子检测及基因分型
Molecular Detection and Genotyping of Bovine Viral Diarrhea Virus in Some Areas of China from 2022 to 2024
摘要
Abstract
Bovine viral diarrhea virus(BVDV)is one of the major pathogens responsible for sig-nificant economic losses in cattle herds,particularly affecting the health and development of calves.This study aimed to establish a universal SYBR Green Ⅰ-based real-time quantitative PCR(qPCR)assay for BVDV detection and to investigate the epidemiology,genotypes,and subtypes of BVDV in samples collected from free-range cattle herds in selected regions of China.A univer-sal BVDV SYBR Green Ⅰ qPCR assay was developed based on the 5'untranslated region(5'UTR)sequences of various BVDV genotypes obtained from GenBank.Using this method,a total of 712 serum samples from free-range cattle,as well as tissue samples from calves exhibiting symptoms such as coughing,fever,and diarrhea collected between June 2022 and December 2024,were tested and subjected to phylogenetic analysis.Additionally,virus isolation,indirect immunofluo-rescence assay(IFA),and hematoxylin-eosin(HE)staining were performed on calf samples.The established BVDV SYBR Green Ⅰ qPCR assay demonstrated high sensitivity,strong specifici-ty,good linearity,and excellent reproducibility.The positive detection rate in serum samples was 19.10％,and the BVDV-24GS strain was successfully isolated.Phylogenetic analysis of the 5'UTR gene sequences revealed that the BVDV strains belonged to subtypes 1c,1m,1o,1i,1q,1b,and 2.These findings indicate the successful development and application of a universal BVDV SYBR Green Ⅰ qPCR assay,and the presence of varying degrees of BVDV infection among free-range cattle in parts of China.The identification of viral genotypes enriches the molecular ep-idemiological data of BVDV and provides a scientific basis for effective disease control,vaccine development,and optimization of immunization strategies.关键词
牛病毒性腹泻病毒/SYBR Green Ⅰ实时荧光定量PCR/分子检测/基因分型鉴定Key words
bovine viral diarrhea virus/SYBR Green Ⅰ real-time quantitative PCR/molecular de-tection/genotype identification分类
畜牧业引用本文复制引用
安乐乐,任晓婷,蓝秋菊,莫永利,曹小安,丁玉林,马忠仁,马晓霞..2022-2024年中国部分地区牛病毒性腹泻病毒分子检测及基因分型[J].畜牧兽医学报,2025,56(10):5328-5334,7.基金项目
甘肃省自然科学基金(23JRRA715) （23JRRA715）
西北民族大学引进人才科研项目(xbmuyjrc202225) （xbmuyjrc202225）
