西南农业学报2025，Vol.38Issue(9)：1953-1963,11.DOI:10.16213/j.cnki.scjas.2025.9.014
根际土壤真菌对旱种水稻生长及稻瘟病抗性的影响
Effects of rhizosphere soil fungi on paddy rice growth and blast disease resistance in dryland cultivation
摘要
Abstract
[Objective]The study aimed to investigate the diversity and functions of rhizosphere soil fungi in upland rice and paddy rice grown under drought conditions.[Method]Fungi in rhizosphere soil were isolated and cultured by single spore,and ITS sequences of the fungi were amplified by PCR.Seven isolated rhizosphere soil fungi Massarina igniaria,Cumuliphoma omnivirens,Phialocephala humicola,Gongronella butleri,Metarhizium robertsii,Absidia pseudocylindrospora and Paecilomyces marquandii were selected to inoculate upland rice and paddy rice seedlings growing in dryland condition.Seed germination rates and plant heights were measured.Concurrently,the rice variety Lijiangxintuan-heigu(LTH)was inoculated with the two rhizosphere soil fungi M.eburnea and Stagonosporopsis spp,followed by inoculation with the fungus Magnaporthe oryzae strain GUY 11.Lesion lengths on rice leaves were measured,the expression of the MoPot2 gene was detected by Real-time PCR,and the disease resistance of rice was evaluated.[Result]Seventy-one rhizosphere soil fungi belonging to 17 genera and 54 species were isolated.Among them,Fusarium,Penicillium and Purpureocillium were the dominant groups in the rhizosphere soil of paddy rice and upland rice.The fungi diversity of different varieties in rhizosphere soil was Nipponbare＞Dayakougu＞Acuce＞Laozhaiheigu＞Lengquangu＞Xiang-tanggu.It showed that the seven isolated rhizosphere soil fungi M.igniaria,C.omnivirens,P.humicola,G.butleri,M.robertsii,A.pseudocylin-drospora and P.marquandii promoted the growth of paddy rice Acuce,Nipponbare,LTH,as well as upland rice Xiangtanggu,Laozhaiheigu,and Lengquangu,while inhibiting the growth of upland rice Dayakougu.M.igniaria promoted the resistance of LTH to rice blast,while C.om-nivirens decreased the resistance of LTH to rice blast.It also showed that treatment of M.igniaria and C.omnivirens induced the expression of salicylic acid receptor gene OsNPR1 and phytoalexins sakuranetin biosynthesis gene OsNOMT in the paddy rice LTH.[Conclusion]The di-versities of rhizosphere soil fungi regulate plant growth and resistance during rice seedlings growing in drought condition,and promote paddy rice adapting to dryland cultivation.关键词
水稻/陆稻/旱地种植/根际土壤/真菌/多样性Key words
Paddy rice/Upland rice/Dryland cultivation/Rhizosphere soil/Fungi/Diversity分类
农业科技引用本文复制引用
杨傲,彭晟,郭力维,程在全,杜云龙,杨艳芬,张晓燕,陈欣然,左晨熙,张艳梅,刘海燕,李葵秀,刘丽..根际土壤真菌对旱种水稻生长及稻瘟病抗性的影响[J].西南农业学报,2025,38(9):1953-1963,11.基金项目
中央引导地方科技发展资金自由探索类基础研究项目(202407AA110006) （202407AA110006）
国家自然科学基金项目(32260085,32460083) （32260085,32460083）
国家重点研发计划(2023YFD1400204) （2023YFD1400204）
云南省应用基础研究计划重点项目(202301AS070082) （202301AS070082）
云南种子种业联合实验室项目(202205AR070001-01) （202205AR070001-01）
