烟草科技2025，Vol.58Issue(10)：19-32,14.DOI:10.16135/j.issn1002-0861.2024.0806
纳米水凝胶缓释烟碱干预帕金森病模型小鼠作用及机制
Intervention effect and mechanism of sustained nicotine release by nano-hydrogel using a mouse Parkinson's disease model
曹芸 1管明婧 1李延岩 1陈韦剑 1周顺 2张劲 1王孝峰 1张晓宇 1田慧娟 1李林 3张亚平 2付硕1
作者信息
- 1. 安徽中烟工业有限责任公司 国家烟草专卖局(中国烟草总公司)燃烧热解研究重点实验室,合肥市高新区永和路399号 231200
- 2. 安徽中烟工业有限责任公司 国家烟草专卖局(中国烟草总公司)燃烧热解研究重点实验室,合肥市高新区永和路399号 231200||安徽中烟工业有限责任公司 安徽省气溶胶解析调控及生物效应重点实验室,合肥市高新区永和路399号 231200
- 3. 厦门大学柔性电子(未来技术)研究院,福建省厦门市思明路422号 361005
- 折叠
摘要
Abstract
In order to decrease the metabolic rate and to extend the action of nicotine,and to explore the intervention effect and mechanism of sustained nicotine release on Parkinson's diseased mice(PD mice),a nano hydrogel for loading nicotine(Nic@NHG)was established using a phase transition microemulsion method,and the nicotine loading ratio and sustained release efficiency using the animal model were studied.Animal behavior analysis was conducted to evaluate the improvement effect of sustained nicotine release on PD mice.Molecular biology methods were employed to analyze the expression levels and phosphorylation status of key proteins in signal pathways to elucidate the molecular mechanism of intervention from Nic@NHG's in Parkinson's disease(PD).The results showed that:1)Sodium alginate nano hydrogel could effectively load and sustainably released nicotine,and the loading rate exceeded 90%for nicotine with mass concentrations of 1.0,1.5 and 2.0 mg/mL.The effective duration of sustained release of nicotine at different mass concentrations in Nic@NHG exceeded 6 hours,and the blood metabolic duration was nearly 12 hours,approximately twice of that in the nicotine system.2)Nic@NHG effectively alleviated the anxiety and improved muscle symptoms of the PD mice,with all major indicators showing significant improvement compared to the nicotine system,and the intervention effect was dose dependent.The intervention effect at 2.0 mg/mL was significantly better than that at 1.0 mg/mL,and the intervention effects at the loading concentrations of 2.0 and 3.0 mg/mL were similar.3)Nic@NHG effectively inhibited inflammatory reactions and neuronal apoptosis in PD mice,demonstrating a significantly better inhibitory effect than the nicotine system.4)Nic@NHG effectively inhibited abnormal phosphorylation in PI3K/Akt and JAK2/STAT3 signal pathways of PD mice,upregulated Survivin,downregulated Caspase-3 and proinflammatory factors such as IL-6,TNF-α,iNOS,and MCP-1,and the effects were significantly better than those of the nicotine system,which was close to the experimental results of normal mice.Compared to nicotine,Nic@NHG further inhibited the abnormal phosphorylation in PI3K/Akt and JAK2/STAT3 signal pathways,regulated the expression levels of key proteins,thereby alleviated the anxiety and improved muscle symptoms in PD mice.关键词
烟碱/缓释/帕金森病小鼠/PI3K/Akt信号通路/JAK2/STAT3信号通路/纳米水凝胶Key words
Nicotine/Slow release/Parkinson's disease mice/PI3K/Akt signal pathway/JAK2/STAT3 signal pathway/Nano hydrogel分类
轻工业引用本文复制引用
曹芸,管明婧,李延岩,陈韦剑,周顺,张劲,王孝峰,张晓宇,田慧娟,李林,张亚平,付硕..纳米水凝胶缓释烟碱干预帕金森病模型小鼠作用及机制[J].烟草科技,2025,58(10):19-32,14.
