烟草科技2025，Vol.58Issue(10)：58-66,9.DOI:10.16135/j.issn1002-0861.2024.0750
种子携带镰刀菌对烟苗镰刀菌根腐病发生的影响
Effects of Fusarium spp.carried by tobacco seeds on root rot occurrence of tobacco seedlings
摘要
Abstract
In order to determine the status of tobacco seeds carrying Fusarium spp.,Fusarium strains were isolated and purified from coated and naked seeds by petri dish culture method,followed by morphological and molecular analyses and the pathogenicity determination.Through moist germination of tobacco seeds on sterile filter paper and fungal plate culture in water agar,the fungus bearing rate of coated and naked seeds were found to be 0-10.00%and 5.00%-85.00%respectively,and the proportion of Fusarium spp.in coated seeds of the isolated fungus strains was significantly lower than that in naked seeds.The identification results showed that the tested tobacco seeds carried Fusarium oxysporum,Fusarium graminearum,Fusarium incarnatum in Fusarium incarnatum-equiseti species complex,Fusarium citri,Fusarium ipomoeae,Fusarium lacertarum,Fusarium verticillioides in Gibberella fujikuroi species complex,Fusarium fujikuroi,and Fusarium andiyazi.The 21 purified Fusarium strains were all pathogenic to Zhongyan 100 and LY1306 seedlings,and the disease indexes were 21.11-100.00.关键词
烟草/种子带菌/包衣种子/裸种/镰刀菌/致病性Key words
Tobacco/Seed carrying bacteria/Coated seed/Naked seed/Fusarium spp./Pathogenicity分类
农业科技引用本文复制引用
邱睿,杨晋燕,李伟观,张盈盈,李小杰,李彩虹,李淑君..种子携带镰刀菌对烟苗镰刀菌根腐病发生的影响[J].烟草科技,2025,58(10):58-66,9.基金项目
中国烟草总公司重大科技项目"基于生物防治的黄淮烟区烟草有害生物综合治理技术集成与模式构建"[110202101051(LS-11)] （LS-11）
河南省农业科学院科技创新团队"烟草主要病虫害绿色防控关键技术研究与应用"(2024TD42) （2024TD42）
河南省农业科学院自主创新项目"烟草茄病镰刀菌致病基因筛选研究"(2024ZC042) （2024ZC042）
中国烟草总公司揭榜挂帅项目"黄淮平原生态区粮烟融合绿色防控综合治理技术体系研究与应用"[110202201026(LS-10)]. （LS-10）
